河北地区汉族人非胰岛素依赖型糖尿病眼底病变与ACE基因多态性的相关性研究

RELATIONSHIP BETWEEN ANGIOTENSIN COVERTING ENZYME GENE POLYMORPHISM AND DIABETIC RETINOPATHY IN NIDDM PATIENTS OF HEBEI PROVINCE

目的 探讨血管紧张素Ⅰ转换酶（ＡＣＥ） 基因插入／ 缺失（Ｉ／Ｄ） 多态性与非胰岛素依赖型糖尿病（ＮＩＤＤＭ） 及并发眼底病变的关系。方法 以ＡＣＥ基因１６ 内含子的１ 个２８７ｂｐ 的Ａｌｕ 序列为多态性标志，用ＰＣＲ方法扩增基因片段，６ ％ 非变性聚丙烯酰胺凝胶电泳检测ＰＣＲ 产物。结果１４９ 例ＮＩＤＤＭ 与１００ 例正常人基因型频率与等位基因频率差异无显著性。ＤＤ 型与Ｄ 等位基因在ＤＲ（ ＋ ） 组出现频率高，ＤＲ（ ＋ ） 组与ＤＲ（ － ） 组相比，基因型及等位基因分布差异具显著性。结论 ＡＣＥ 基因多态性与ＮＩＤＤＭ

ObjectiveTo clarify the relationship between ACE gene I/D polymorphism and development of diabetic retinopathy in non insulin dependent diabetic mellitus (NIDDM). MethodsA fragment of 287 bp Alu sequence in intron 16 of ACE gene was used as I/D polymorphic marker. After PCR amplification of DNA fragment, 6 % nondenatured polyacrylmide gel electrophoresis was undertaken to analyse the PCR products. ResultsThe distribution of genotype and frequency of the two alleles in NIDDM (149 cases) were very similar to the normal control (100 cases). However,the DD genotype and D allele had higher frequency in NIDDM with retinopathy than without.ConclusionThe I/D polymorphism of ACE gene may attribute to development of diabetic retinopathy in NIDDM in Hans of Hebei province.