嘉陵江瓦氏黄颡鱼卵巢生物化学周年变化的研究

Studying on Annual Change of Female Pelteobagrus vachelli＇s Biochemistiry in Jia-Ling River

本实验通过对瓦氏黄颡鱼组织中蛋白质的含量、AKP／ACP活性和RNA／DNALL率的测定，研究了瓦氏黄颡鱼卵巢发育过程中组织化学周年变化的规律。实验结果表明，卵巢和肝脏中蛋白质的含量随着卵巢发育而逐渐增加，卵巢中的蛋白质在产卵期达到最大值131．93±2．66g／ml，之后下降；而肝脏中的蛋白质在4月中旬达到最大值134．52±1．44g／ml，之后下降。卵巢和肝脏中AKP和ACP活性随着卵巢发育逐渐升高，在4月中旬分别达到最大值。在产卵期降到最小值，之后逐渐上升。卵巢中RNA／DNA比率随着卵巢的发育逐渐增大，在5月中旬卵巢成熟时达到最大值12．71±3．10。6月份开始，RNA／DNA比率开始下降，在8月份时m现上升，10月份以后没有显著性的变化。肝脏中RNA／DNA比率从1月份上升到5月份的最大值3．15±0．33，自6月份开始后，出现下降趋势。这表明，4月中旬至5月初为瓦氏黄颡鱼性腺发育旺盛阶段，5月中旬至7月中旬为繁殖季竹，8-9月为退化修复期。

In this paper, the annuation discipline of organizational AKP, ACP activities and RNA/DNA ratio were studied for probing into the breeding cycle of Pelteobagrus vachelli. The protein content in ovary and liver was gradually increased with the gonad development, which in ovary had rased f the maximum 131.93~2.66g/ml in May,then decreased. The AKP and ACP activities in both ovary and liver were gradually increased with the ovary development, which reached to the maximum in mid-April,then rasied. The RNA/DNA ratio in ovary was gradually increased with the ovary development, which reached the maximum 12.71＋3.10 in the mid-May, it started to decline in June. RNA/DNA ratio in liver reached to the maximum 3.15~0.33 ,and then showed a downward trends from early June. The results showed that the ovary of Pelteobagrus vachelli was in flourish phase from mid-April to mid-May and the breeding season was between mid-May and mid-July. The degradation and rehabilitation period was from August to September.