TIMP-1水平在IgA肾病患者血清中的变化及意义

The Level of TIMP-1 in the Serum of Patients with Different Pathologic Stages of Primary IgA Nephropathy or Renal Failure Caused by Primary Glomerulonephritis

目的 观察不同病理类型的IgA肾病患者和由原发性肾小球肾炎发展至肾衰竭的患者血清中金属蛋白酶组织抑制物-1（tissue inhibitor of metalloprotei-nase-1,TIMP-1）水平的变化.方法 将经肾活检确诊的原发性IgA肾病患者分组,并设立慢性肾衰竭（chronic renal failure,CRF）患者组和正常对照（normal control,NC）组,观察各组间TIMP-1水平的表达情况,数据用（x±s）表示,用方差分析进行统计学处理,P《0.05为有统计学意义.结果 肾衰竭组、IgA肾病重度病理改变组及中度病理改变组血清中TIMP-1水平较正常对照组明显增高（P《0.05）,且肾衰竭组和IgA肾病重度病理改变组血清中TIMP-1水平又较中度病理改变组显著增高（P均《0.01）,而IgA肾病轻度病理改变组患者血清中TIMP-1水平与正常对照组相比无统计学意义.结论 TIMP-1在肾衰竭组、IgA肾病重度病理改变组及中度病理改变组血清中水平明显增高,提示TIMP-1与细胞外基质（（extracelluar matrix,ECM）的增加以及肾脏的纤维化密切相关,它可能通过抑制肾小球细胞外基质的降解过程而导致ECM积聚,从而导致基质纤维化和肾小球硬化.

Objective To study the level of tissue inhibitor of metalloproteinase-1（TIMP-1） in the serum of patients with different pathologic stages of primary IgA nephropathy or renal failure caused by primary glomerulonephritis.Methods Patients undergoing primary IgA nephropathy with renal biopsy were divided into three groups according to pathologic stages.There were also the chronic renal failure（CRF） group and the normal control（NC） group.The TIMP-1 levels of each recipients were detemined by enzyme-linked immunlsorbent assay（ELISA） two-sides antibody.Data are expressed as mean valu（-x±s）EM and compared with individual groups using analysis of variance,value of P0.05 was regarded for statisticl significance.Results TIMP-1 was obviously higher in the renal failure group as well as the severe change group and the moderate change group than the normal contral group（P0.05）,and the TIMP-1 was significantly higher in the renal failure group and the severe change group than the moderate change gourp（P0.01）.But the comparison of the light change group and that in the normal contral group makes no statistic sense.Conclusion In the development of extrcelluar matrix（ECM） and renal sclerosis,there were a close relationship between in the TIMP-1 and the decomposition of ECM among the patients.The result demonstrated that increased expression of TIMP-1 may play a role in the development and progression of renal fibrosis in patients with IgA nephropathy through inhibiting the decomposition of ECM and thus bringing about acculmulation of ECM.