力竭性运动时大鼠脑组织自由基产生及氧化、抗氧化能力的动态观察

Dymanic Observation of Production of Free Radical and Oxidative、Antioxidative Capability of Brain in Rats During Exhausting Exercise

以大鼠递增负荷力竭性运动为模型，利用低温电子自旋共振（ＥＳＲ）技术，分别测定安静时、运动过程中、运动后即刻、运动后恢复期３０ｍｉｎ、２ｈ、４ｈ及８ｈ脑组织的氧自由基（ＯＴＲ）信号强度，同时测定ＳＯＤ活力和ＭＤＡ含量。结果显示：脑组织在运动过程中ＯＦＲ信号强度逐渐增加，并具有运动强度依赖的阶段性，恢复期４小时达到高峰；ＳＯＤ活性运动时无明显改变，恢复期２ｈ升至峰值；脂质过氧化水平（ＭＤＡ）在整个运动过程中及恢复期无显著变化。提示该运动模型所引起的脂质过氧化尚未累及中枢神经系统，并且脑组织可能存在重要而有效的抗氧化体系。

Taking incremental exercise to exhaustion and using low temperature electron spin respmsmvr(ESR ) technique we measured the brain intensity of OFR species, SOD activity, MDA content at rest during exercise immediately 30min 2h 4h 8h post exercise The results showed that the intensity of OFR species increased gradually during exercise with intensity-dependent manner,and reached its highest level 4h post-exercise ;SOD activity did not change during exercise and increased significantly 2h post- exercise; MDA content kept constant during the whole exercise and recovery period which suggested that lipoperoxidation induced by the present exercise model did not involve in central nermous system and a very importana effective antioxidative system may be existed in the brain.