摘要
目的探讨鸡尾酒抗体甲基酰基辅酶A消旋酶(AMACR)、高分子量角蛋白抗体(HMWCK)和p63在前列腺腺癌(Pca)、高级别前列腺上皮内瘤(HGPIN)及良性前列腺增生(BPH)组织中的表达及意义。方法收集45例Pca、40例HGPIN和45例BPH标本,按常规方法石蜡包埋,制作蜡块,进行苏木素-伊红染色及AMACR、HMWCK和p63鸡尾酒抗体双重免疫组化染色。结果 Pca中AMACR阳性率为84.4%;HGPIN中AMACR阳性率为82.5%,与Pca中比较差异无统计学意义(P>0.05);BPH中AMACR阳性率为24.4%,与Pca和HGPIN比较差异均有统计学意义(P<0.05)。HMWCK和p63在高分化Pca中均呈阴性,15.6%中分化和低分化Pca的癌巢周围可见,HGPIN和BPH中均呈阳性表达,但其分布方式不同,在HGPIN中呈连续或间断性分布,从HGPIN至癌移行腺体中逐渐减少至消失,在BPH中呈连续性分布。HMWCK和p63在Pca的表达与HGPIN和BPH比较,差异有统计学意义(P<0.05)。结论鸡尾酒抗体AMACR、HMWCK和p63对诊断高分化Pca有较高的特异性和敏感性,同时对高分化Pca和异型病变的鉴别诊断有价值。
Objective To investigate the expression and significance of a triple cocktail antibodies,AMACR,HMWCK and p63,in human prostatic adenocarcinoma(Pca),high grade prostatic intraepithelial neoplasia(HGPIN)and benign prostatic hyperplasia(BPH). Methods Forty-five Pca,40 HGPIN and 45 BPH specimens were collected.Conventional paraffin-embedding and serial section tissues were used to perform HE and immunohistochemical cocktail staining(AMACR/HMWCK/p63).The expression of AMACR/HMWCK/p63 cocktail antibodies was detected by immuohistochemisitry staining(cocktail antibody double staining).The expression of the AMACR/HMWCK/p63 and the relationship with histopathologic classification were analyzed. Results The positive rate of AMACR in Pca was 84.4%,and the positive rate of AMACR in HGPIN was 82.5%,the difference was not statistical(P0.05).While the positive rate of AMACR in BPH was 24.4%,showing significant difference compared with those in Pca and HGPIN(P0.05).The percentage of AMACR staining intensity in PCas(84.4%) were significantly higher than in HGPINs(82.5%)and BPHs(24.4%)(P0.01).The expression of HMWCK/p63 was significantly higher in BPHs and HGPINs than PCas(P0.01).There was no statistically significant difference of HMWCK/p63 staining between BPH and HGPIN,and its expression was unrelated to Gleason grading of PCa(P0.05).The expression of HMWCK and p63 showed statistical difference between Pca,HGPIN and BPH(P0.05). Conclusion AMACR/HMWCK/p63 expression is different in Pca,HGPIN and BPH.AMACR/HMWCK/p63 cocktail staining is useful for the diagnosis of well differentiated adenocarcinoma,benign and malignant prostate disease.
出处
《中国全科医学》
CAS
CSCD
北大核心
2011年第8期867-869,共3页
Chinese General Practice
关键词
前列腺肿瘤
腺癌
抗体
免疫组织化学
Prostatic neoplasms
Adenocarcinoma
Antibodies
Immunohistochemistry