摘要
目的探讨舒洛地特对大鼠脑缺血再灌注后脑组织的保护作用及机制。方法将72只Wistar大鼠随机分为3组即假手术组、模型组、舒洛地特干预组,采用改良Zea Longa[1]线栓法制备大鼠大脑中动脉局灶性脑缺血模型,应用免疫组化法检测肿瘤坏死因子-а(TNF-а)及C-反应蛋白(CRP)的表达。结果大鼠局灶性脑缺血再灌注后缺血脑组织中TNF-a及CRP含量增加(P<0.01)。与模型组相比较,舒洛地特组TNF-a及CRP的含量减少,脑组织肿胀减轻,死亡神经元明显减少,神经功能缺损评分降低(P<0.05)。结论舒洛地特对大鼠脑缺血再灌注损伤有一定的保护作用,其机制可能是多方面的。
ObjectiveTo investigate the neuroprotective effects and mechanism of sulodexide on focal cerebral ischemia and ischemia-reperfusion injury in rats.MethodsSeventy-two Wistar rats were randomly divided into three groups,sham-operative group,model group and sulodexide group.The model of middle cerebral artery occlusion（MCAO） was made by the method of modified Zea Longa.The expressions of TNF-α and CRP were detected by immunohistochemical analysis.ResultsThe expressions of TNF-α and CRP in the ischemic territory increased after cerebral ischemia（P0.01）.Compared with the model group,the expression of TNF-α and CRP decreased in sulodexide group,the swelling of cerebral lightened,the number of death nerve cell decreased,and Longa＇s score reduced（P0.05）.ConclusionSulodexide could protect the cerebral after focal cerebral ischemia,the mechanism is complicated.
出处
《中国实用神经疾病杂志》
2011年第3期30-32,共3页
Chinese Journal of Practical Nervous Diseases
