氯胺酮对感染性休克大鼠脑组织中热休克蛋白70的影响

目的观察氯胺酮对感染性休克大鼠血流动力学、7d生存率以及脑组织中热休克蛋白70的影响。方法取健康成年雄性(SD)大鼠60只,随机分为对照组、CLP组和氯胺酮组,各组20只。采用盲肠结扎穿孔(CLP)法复制感染性休克模型(CLP组),氯胺酮组于制模前腹腔给予80mg/kg。采用颈总动脉穿刺置管,持续监测平均动脉压(MAP);观察各组大鼠7d存活时间及存活率;用免疫组化法测定脑组织热休克蛋白70(HSP70)阳性表达。结果对照组各时间点的MAP趋于稳定,CLP组术后MAP进行性下降,氯胺酮处理能逆转MAP下降;CLP组大鼠存活率(0)显著低于对照组(100%)及氯胺酮组(40%),差异均有显著性(P<0.05);CLP组HSP70阳性产物灰度值显著低于对照组,但显著高于氯胺酮组(P<0.01)。结论氯胺酮可以提高大鼠7d生存率具有抗感染性休克作用,可能与增加脑组织中热休克蛋白70的表达有关。

Objective To investigate the effects of ketamine on hemodynamics, survival rate and expression of heat shock protein70 in septic shock rats. Methods Sixty SD rats were randomly divided into control group , CLP group and ketamine group.Cecal ligation and puncture（CLP） was used to reproduce septic shock model. In ketamine group, the rats received intraperitoneal injection of 80mg/kg ketamine 20 minutes before shock. The carotid artery catheterization was cannulated to monitor mean arterial pressure; the survival time and survival rate were observed in each group; expression of heat shock protein70 （HSP70） was assessed by immunohistochemistry method. Results CLP produced progressive hypotension. The hemodynamic responses were reversed in ketamine treated animals. The survival rate was lower in CLP group （0） compared with control group （100%） and ketamine group （40% ） , the differences were significant （both P〈0.05）. Positive expression of HSP70 in CLP group was lower than control group, while higher than ketamin group（both P〈0.01）. Conclusion Ketamine can increase the survival function with anti-septic shock, which may increase the brain tissue of heat shock protein 70 expression.