进口废旧物品携带微生物状况初步研究

Preliminary study on microorganisms carried by entry renewable waste

目的调查进口废旧物品携带微生物状况。方法按集装箱采样,用试纸片法对样本微生物进行细菌总数、大肠杆菌、沙门氏菌计数,用致病菌增菌检测试剂盒、免疫层析检测片、胶体金快速检测试剂盒和全自动微生物分析系统进行致病菌检测。结果检测93份样本,金黄色葡萄球菌阳性率为61.29%,沙门菌阳性率为33.33%,腊样芽胞杆菌阳性率为72.04%,副溶血弧菌58.06%,致泻大肠埃希菌29.03%。检出铜绿假单胞菌、杀鲑气单胞菌、温和气单胞菌、溶血葡萄球菌、土拉热弗朗西菌、肺炎链球菌、麻疹孪生球菌、志贺菌、粘质沙雷菌、阴沟肠杆菌、红斑丹毒丝菌等致病菌、条件致病菌及非致病菌。在93份样本中均未检测出出血性大肠杆菌O157:H7,霍乱弧菌O139二种致病菌。结论进口废旧物品中致病微生物的检出率较高,对从业人员的身体健康和环境危害的评价,有待深入研究。

Objective To investigate microbiological status carried by immigration recyclable waste.Methods Total number of bacteria,E.coli,Salmonella of samples were detected by test paper disc method,and the pathogens detection with bacterial pathogens detection kit、rapid immunochromatography assay、rapid colloidal gold assay and automated microbial analysis system.Results The positive rate of Staphylococcus aureus was 61.29% in 93 samples,and the positive rate of Salmonella was 33.33%,Bacillus cereus 72.04%,Vibrio parahaemolyticus 58.06%,Escherichia coli 29.03%.Pathogens,conditions and non-pathogenic bacteria detected in 93 samples included Pseudomonas aeruginosa、Aeromonas salmonicida、Aeromonas sobria、Staphylococcus haemolyticus、Francisella tularensis、Streptococcus pneumoniae、Gemella morbillorum、Shigella、Serratia marcescens、Enterobacter cloacae、Erysipelothrix rhusiopahiae.Both of Escherichia coli O157： H7 and Vibrio cholerae O139 bacteria were not detected in 93 samples.Conclusions The detection rate of pathagenic microorganisms was high.It is necessary to study on the health and environmental hazard assessment.