高三尖杉酯碱和异三尖杉酯碱对人急性早幼粒白血病细胞凋亡的诱导作用

Apoptosis Inductive Effect of Homoharringtonine and Isoharringtonine on Human Acute Promyelocytic Leukemia HL-60 Cells

目的研究高三尖杉酯碱和异三尖杉酯碱对人早幼粒白血病细胞ＨＬ－６０凋亡的影响。方法采用流式细胞术、ＤＮＡ凝胶电泳、光镜及透射电镜等方法。结果证明高三尖杉酯城及异三尖杉酯碱能快速、显著地诱导ＨＬ－６０细胞的凋亡，其作用有明显的浓度及时间依赖性。高三尖杉酯碱的活性明显高于异三尖杉酯碱。结论高三尖杉酯碱及异三尖杉酯碱皆可诱导ＨＬ－６０细胞凋亡，但高三尖杉酯碱的生物活性明显高于异三尖杉酯碱。

To study the effects of homoharringtonine and isoharringtonine on the induction of apoptosis on human promyelocytic leukemia HL-60 cells. Methods By using flowcytometry,DNA gel electrophoresis, microscopy and electron microscopy. Results it wasdemonstrated that homoharringtonine and isoharringtonine can induce apoptosis of HL-60cells significantly and rapidly. The activity of homoharringtonine was higher than that of isoharringtonine,and it was time and concentration dependent. Conclusions Both homoharringtonine and isoharringtonine could induce apoptosis of HL-60 cells in terms of morphology,and DNA ladder assay. The activity of homoharringtonine was much higher than that ofisoharringtonine.