摘要
目的:观察白藜芦醇作用前后TRAIL对人髓系白血病KG-1a细胞的细胞毒作用的变化。方法:流式细胞仪检测KG-1a细胞表面CD34和CD38的表达,二甲氧唑黄(XTT)细胞增殖及细胞毒性检测试剂盒检测白藜芦醇作用前后TRAIL对KG-1a细胞增殖的影响,AnnexinV-FITC/PI染色流式细胞仪检测细胞凋亡变化。流式细胞仪检测白藜芦醇作用前后KG-1a细胞表面TRAIL死亡受体表达变化。结果:人髓系白血病KG-1a细胞CD34+CD38-占(58.67±2.87)%,101 000 ng/ml的TRAIL对KG-1a细胞增殖无明显影响,但对白藜芦醇作用后的KG-1a细胞的增殖有明显抑制作用,白藜芦醇能促进TRAIL诱导KG-1a细胞凋亡,并能上调KG-1a细胞表面TRAIL死亡受体DR5的表达。结论:白藜芦醇能增强TRAIL对人髓系白血病KG-1a细胞的细胞毒作用,其机制可能与白藜芦醇上调KG-1a细胞表面TRAIL死亡受体DR5的表达有关。
Objective:To explore the cytotoxicity of TRAIL to human acute promyeloblastic leukemia KG-1a cells treated with resveratrol.Methods:The expression of CD34 and CD38 on the surface of KG-1a cells was detected by flow cytometry;The effects of various concentration TRAIL on proliferation of KG-1a cells treated with or without resveratrol were analyzed by XTT cell proliferation and cytotoxicity assay kit;The changes of apoptosis in KG-1a cells treated with or without resveratrol induced by TRAIL were detected by flow cytometry through the staining of AnnexinV-FITC/PI;The effects of resveratrol on the expression of DR4/5 on the surface of KG-1a cells were detected by flow cytometry.Results:The CD34+CD38-percent age of KG-1a cells was(58.67±2.87)%;101 000 ng/ml of TRAIL had no effects on the proliferation of KG-1a cells,while could inhibit the proliferation of KG-1a cells treated with resveratrol;Moreover,resveratrol could enhance the apoptosis of KG-1a cells induced by TRAIL and up-regulate the expression of TRAIL death receptors DR5 on the surface of KG-1a cells.Conclusion:Resveratrol could enhance the cytotoxicity of TRAIL to human promyeloblastic leukemia KG-1a cells,perhaps because of the up-regulation of TRAIL death receptor DR5 on the surface of KG-1a cells treated with resveratrol.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2011年第3期217-219,227,共4页
Chinese Journal of Immunology
基金
国家自然科学基金(No.30973454)资助