摘要
目的:分析可诱导共刺激分子(ICOS)可溶性融合蛋白(ICOSIg)能否向不成熟DCs传递逆向信号及其性质。方法:以流式细胞仪结合特异性抗体检测DCs表型分子改变;以ELISA检测培养上清细胞因子变化;以RT-PCR检测各组DCs细胞内细胞因子及受体、趋化因子等mRNA表达水平。结果:ICOSIg或膜锚定ICOS作用于不成熟DCs,均可诱导其高表达MHC-Ⅱ、CD80、CD86和CD83等表型分子;促进DCs特异性分泌IL-6。结论:ICOS作用于不成熟DCs表面的ICOSL可以向DCs细胞传递逆向信号,诱导DCs细胞高分泌IL-6,同时其表面重要的表型分子也上调,可能参与了DCs细胞免疫功能的调节,其信号转导机制可能涉及p38-MAPK通路。
Objective:To determine whether ICOS could deliver any reverse signals through ICOSL expressed on mouse-derived immature dendritic cells(DCs) and to detail their possible characteristics.Methods:The surface phenotype molecules of DCs were determined by flow cytometry and the variation of cytokines was detected by ELISA.Real-time PCR was used for the evaluation of mRNA levels of cytokines,chemokines and their receptors.Results:High expression of MHC-Ⅱ,CD80,CD86 and CD83 was induced on the surface of DCs when they were coincubated with ICOSIg or CHO cells expressing membrane-anchored ICOS.More intriguingly,IL-6 was significantly and specifically elevated in these conditions.Conclusion:Our data,for the first time,provide some biological proofs showing that ICOS,through binding ICOSL on the cell surface,can deliver some reverse signals to immature DCs,inducing a specific secretion of IL-6 by activating p38-MAPK signal pathway.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2011年第3期220-227,共8页
Chinese Journal of Immunology