火焰原子吸收光谱法间接测定药剂中的核黄素

Indirect determination of riboflavin in pharmaceuticals by flame atomic absorption spectrometry

本文利用高碘酸钠对相邻羟基氧化作用的专属性,在一定介质中,高碘酸钠与核黄素完全反应后,过量的高碘酸的钠与硝酸铅或者硝酸铜生成沉淀,通过测定Pb2+或者Cu2+,建立了间接测定核黄素含量的方法。铅体系和铜体系测定的相对标准偏差(RSD)分别为4.8%和5.2%,检出限分别为0.6μg.mL-1和0.5μg.mL-1。用铅体系间接原子吸收光谱法测定维生素B2片剂和针剂中的核黄素,测定结果与药典方法基本一致。

In the present work,a flame atomic absorption spectrometric method was developed for indirect determination of riboflavin.An excessive sodium periodate was used to react with riboflavin,and the remaining periodate was precipitated with excessive lead nitrate or copper nitrate.The precipitation,lead periodate or copper periodate formed was separated from the reaction medium by membrane filtration,and then was dissolved in 0.2 mol L-1 nitric acid following the determination of lead or copper by flame atomic absorption spectrometry.The calibration curve was linear over the range of 0.001～0.020 mg mL-1 and 0.0025 ～ 0.0500 mg mL-1;the relative standard deviation（RSD） for determination of 0.005 mg mL-1 riboflavin was 4.8% and 5.2%;and the detection limit was 0.0006 and 0.0005 mg mL-1,respectively,with lead or copper AAS measurement.The method was used for determination of riboflavin in Vitamin B2 tablets and injections.