阻抑高碘酸钾氧化甲基紫褪色光度法测定苯酚

Spectrophotometric determination of trace phenol by its inhibitory effect on the oxidation-decoloration of methyl violet with potassium periodate

基于HAc-NaAc缓冲体系中,反应温度为25℃时,苯酚阻抑高碘酸钾氧化甲基紫的褪色反应,建立了测定苯酚的阻抑褪色光度新方法。该方法最大吸收波长为580 nm,线性范围为0.02μg/mL～2.2μg/mL,检出限为3.8×10-3μg/mL,方法用于环境水中苯酚的测定,相对标准偏差为1.97%～2.25%,回收率为99.5%～100.5%。

A spectrophotometric method for the determination of phenol,which based on its inhibitory effect to the oxidatioin reaction of methyl violet with potassium periodate in HAc-NaAc buffer medium at 25℃,was described.The maximum absorption wavelength was 580nm.Phenol could be determined in the concentration range of 0.02μg/mL～2.2μg/mL,and detection limit of the method is 3.8×10-3μg/mL.The method has been successfully applied for the determined of trace phenol in water samples of environment with the relative standard deviations（RSD） of 1.97%～2.25% and the recovery of 99.5%～100.5%.