纳米纤维固相萃取检测血浆中3-硝基酪氨酸

Nanofibers As A Solid-phase Extraction Sorbent for the Determination of Plasma Free 3-Nitrotyrosine

建立了血浆中3-硝基酪氨酸的检测方法。乙腈沉淀血浆蛋白,上清液经氮气吹干后用荧光剂4-氟-7-硝基-2,1,3-苯并氧杂恶二唑(NBD-F)衍生,纳米纤维固相萃取柱净化后,以0.05 M磷酸二氢钾—甲醇(体积比35:65,pH 3.0)为流动相,在Ex=470 nm,Em=530 nm条件下测定峰响应值。该方法具有较高的灵敏度和准确度,能够用于测定血浆中3-硝基酪氨酸的含量。

A reversed-phase liquid chromatography with fluorimetric detection（HPLC-Flu） method to measure 3-nitrotyrosine in plasma was described,which involved 4-fluoro-7-nitrobenzofurazan（NBD-F） pre-column derivatization followed by a novel electrospun polymer nanofibers packed micro-column cleaning and concentration.The mobile phase was composed of solution（0.05M phosphate buffer-methanol,35：65,v/v） at pH 3.0 adjusted with orthophosphoric acid.The wavelengths of excitation and emission were 470 nm and 530 nm,respectively.The method had high sensitivity and accuracy for the determination of 3-nitrotyrosine levels in plasma.