匀浆法提取-高效液相色谱法测定金银花中的绿原酸

Determination of Chlorogenic Acid Extracted from Flos Lonicerae with High-Speed Homogenization and High Performance Liquid Chromatography

分别用3种前处理方法对金银花中的绿原酸进行提取，用高效液相色谱法进行检测．结果表明，用匀浆法提取所需时间明显少于热回流提取法和超声波提取法，采用80％的甲醇匀浆法提取金银花中的绿原酸，提取液中的绿原酸以WatersXterraRPl8（3．9mm×150mm，5μm）色谱柱为固定相，1％醋酸为流动相，梯度洗脱，流速为0．8mL/min，检测波长345nm．在该色谱条件下，绿原酸质量浓度在5．0～200μg／mL内成良好的线性关系，样品平均加标回收率为118％，相对标准偏差（RSD）为4．5％（n=6），结果令人满意．

In this paper, three sample preparation for determination of chlorogenic acid in flos lonicerae was studied. Test results showed that the time spent on high - speed homogenization extraction was obviously less than the ultrasonic extraction and the hot reflux extraction. The chlorogenic acid was extracted from samples by high - speed homogenization with 80% methanol solution. The extract was separated on a Waters Xterra RP18 column （3.9mm ×150 mm,5 μm） using 1% acetic acid as the mobile phase with gradient elution, the flow rate was 0.8 mL/min. The detection wavelength was 345 nm. Under this condition, the linear of chlorogenic acid was in the range of 5.0--200 μg/mL. The average recoveries of the samples were 118% , and the relative standard deviation was 4. 5% （n = 6）. This method for the extraction and determination of chlorogenic acid in flos lonicerae has good results.