ELISA与TPPA检测梅毒螺旋体的临床对比研究

Clinical comparative study on ELISA and TPPA detectioned of treponema pallidum

目的观察酶联免疫吸附试验(ELISA)与梅毒螺旋体明胶颗粒凝集试验(TPPA)对检测梅毒螺旋体抗体的临床效果。方法对283份血清标本分别进行TPPA法和ELISA法检测,对结果进行分析。结果在对158份不同患者和正常献血者血清的检测中,ELISA法和TPPA法均未检测出阳性反应。125份健康体检者血清用ELISA法检出梅毒抗体阳性13份,阳性率为10.4%;TPPA法检出阳性11份,阳性率为8.8%,2种方法阳性率比较差异无统计学意义(P>0.05),检测总符合率为98.4%。结论 ELISA法对各期梅毒均有较高的敏感性和特异性,易于标准化、规范化,适合大量样本的筛查;TPPA法虽然敏感性、特异性较高,但操作繁琐、耗时长、成本较高,适宜作确认试验。

Objective To observe the effect of enzyme-linked immunosorbent assay（ELISA） and gelatin treponema pallidum particle agglutination test（TPPA） for detection of treponema pallidum.Methods 283 cases serum samples respectively did ELISA and TPPA,and analyzed the results.Results In 158 cases different patients and detection of normal human serum,ELISA and TPPA were not detected in positive law.The serum of 125 cases healthy used ELISA method,syphilis antibody positive rate was 10.4%（13 cases）;Used TPPA method,syphilis antibody positive rate was 8.8%（11 cases）,the difference was not statistically significant（P0.05）,the total coincidence rate was 98.4%.Conclusion ELISA method to the syphilis have a higher sensitivity and specificity and easy standardization,standardization,and suitable for screening large sample;TPPA method,while the sensitivity and specificity are high,but relatively speaking,the operation is more complicated,time-consuming,high cost,suitable for confirmatory test.