响应面法优化牦牛肝蛋白提取工艺研究

Optimization of Extraction Conditions for Protein from Yak(Bos grunniens) Liver by Response Surface Methodology

对牦牛肝中主要营养成分进行测定,然后用单因素和响应面分析法确定牦牛肝蛋白的最佳提取工艺。通过单因素试验选取影响因素与水平,在此基础上采用四因素三水平的响应面分析法,根据回归分析确定最佳提取工艺。试验结果表明:鲜牦牛肝水分含量为70.85%、灰分含量为0.7%、蛋白含量为19.70%、粗脂肪含量为2.92%;最佳提取工艺为:液料比(ml:g)7、碱液浓度0.14 mol·L-1、提取时间68 min、提取温度53℃,采用该条件蛋白提取率可达88.38%。

The contents of nutrient components in liver of yak（Bos grunniens） were determined.The optimum extraction of yak liver protein was determined with the single factor and the response surface analysis.The four factors chosen for the present investigation were based on the results of a single-factor test.Then the optimum extraction process condition was determined based on the regression analysis on the basis of single factor experiment using a 4-factor,3-level response surface methodology.The experiment results showed that moisture,crude ash,crude protein,and crude fat in fresh liver of yak were 70.85%,0.7%,19.70 %,2.92%,respectively.The optimum extraction conditions of yak liver protein were： liquid-solid ratio was 7ml/g,extraction time was 68min,and extraction temperature was 53℃.Under these optimum extraction conditions,the extraction rate of the protein reached 88.38%.