中度嗜盐菌产木聚糖酶发酵条件的研究

Study on Fermentation Conditions of A Moderately Halophilic Bacterium Producing Xylanase

中度嗜盐菌在盐碱环境下生长繁殖,其产生的木聚糖酶也同样具有在盐碱环境下发挥作用的特性。本文对一株中度嗜盐菌的产木聚糖酶活性进行了初步研究。研究包括氮源、液体种子接种量、培养温度、pH值、培养时间等因素对该菌株产木聚糖酶能力的影响。结果表明,最佳培养氮源为蛋白胨;最佳产生木聚糖酶的发酵条件是液体种子接种量为6%,温度为35℃,pH值7,培养时间为4 d。

Moderately halophilic bacteria can grow and propagate under high saline environment,the producing xylanase also has the characteristics of playing roles in the high salinity and alkalinity environment.We studied its activity of xylanase,the effects of nitrogen sources,inoculating quantity of liquid seed,temperature,pH value and culture time.The results showed that the optimal nitrogen source was peptone and the optimum conditions producing the enzyme were as follows： the liquid inoculation of 6%,culture temperature of 35℃,initial pH of 7 and culture time of 4d.