不同等级黄体与水牛胚胎移植受胎率关系的研究

Study On Correlation Between Different Corpus Luteum Level and Pregnancy Rate of Embryo Transfer in Buffalo

[目的]为了解胚胎移植过程中不同等级黄体与水牛胚胎移植受胎率之间的关系,为提高水牛受胎率提供依据。[方法]在水牛胚胎移植过程中,利用B超对受体卵巢进行扫描,确定黄体的有无并根据黄体大小对其分级。黄体直径在1.5 cm以上的定为A级,直径在0.8~1.5 cm之间的定为B级,直径在0.8 cm以下的定为C级。测定受体移植日孕激素水平。受体移植45 d后用B超进行孕检,综合分析研究黄体大小、孕激素水平与移植受胎率之间的关系。[结果]共移植受体水牛234头,A组受胎率为27.27%（12/44）,B组为19.23%（15/78）,C组为22.73%（5/22）,对照组为20.00%（18/90）。[结论]在水牛胚胎移植过程中,黄体大小与受胎率之间没有太大的关系。

【Objective】The objective of this study is to investigate the correlation between the different corpus luteum level and pregnancy rate during the embryo transfer in buffalo and to afford data about improving buffalo pregnancy rate.【Method】During the embryo transferred in buffalo,the ovaries of the receptors were scanned by ultrasound to determine the presence or not of the corpus luteum and to classify them according their size.The corpus luteum with 1.5 cm and more,0.8~1.5 cm,0.8cm or less in diameter were classified respectively as A,B and C grade.The progesterone level of the receptors was also determined in embryo transfer day.Pregnancy determination was performed on the receptors by ultrasound scanning,45 days after the embryo transfer for a comprehensive analysis of the correlation between the size of corpus luteum,the progesterone level and the embryo transfer pregnancy rate.【Result】 In a total of 234 transplant recipients Buffaloes,the pregnancy rate in A,B and C groups were 27.27%（12/44）,19.23%（15/78） and 22.73%（5/22）,respectively.And the control group was 20.00%（18/90）.【Conclusion】In the process of embryo transfer in buffalo,there was no important correlation between the corpus luteum size and the pregnancy rate.