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DDIT4基因在结直肠癌组织中的突变及其临床意义

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摘要 目的探讨DDIT4基因突变在的临床意义。方法应用PCR-直接测序方法检测23份结直肠癌(CRC)、22份结直肠腺瘤(CRA)和18份正常结直肠粘膜组织中DNA损伤诱导转录子4(DDIT4)基因突变情况。结果 CRC标本中共发现两类杂合性突变,均位于DDIT4基因的第2外显子。其中1例为15密码子TCT→ACT,即丝氨酸变为苏氨酸;3例为68密码子GAA→AAA,即谷氨酸变为赖氨酸。22份CRA和18分正常黏膜中均未检测到这两类突变。DDIT4基因在CRC中的基因突变率显著高于CRA和正常组织,P<0.05。结论 DDIT4基因突变在诱导CRC细胞凋亡过程中发挥一定作用。
作者 唐慧 李丽 王金丽 郭强 严新民
机构地区 云南省第一人民医院
出处 《山东医药》 CAS 北大核心 2011年第7期52-54,共3页 Shandong Medical Journal
基金 云南省社会发展项目资助(2008CD203)
关键词 结直肠癌 结直肠腺瘤 DNA损伤诱导转录子4 基因突变
分类号 R735.3 [医药卫生—肿瘤]
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参考文献16

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山东医药
2011年 第7期

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