土壤中砷氧化菌的生理生化及转化砷特性研究

Arsenite Transformation Characteristics and Molecular Identification of Arsenic-oxidizing Bacteria Isolated from Soil

[目的]通过外加砷源驯化肇庆市鼎湖山自然保护区土壤中细菌,研究砷氧化菌的生理生化及转化砷特性。[方法]采用富集、稀释平板、硝酸盐漫过、生理生化指标的测定等。[结果]从中分离、鉴定出具有氧化砷功能的产碱杆菌和土壤杆菌2种菌株。[结论]这2种菌株最适氧化砷温度为30℃,最适氧化砷pH为9。培养基中乳酸钠浓度对菌株氧化砷有一定的影响。

[Objective] Through domesticated bacteria from the applied arsenic source soil in Dinghushan Nature Reserve of Zhaoqing City,physiological,biochemical and transformation characteristics of arsenic oxide bacteria were studied.[Method]The methods of concentration,plates serial dilution,silver nitrate overflowed,physiological characteristic were adopted.[Result]They were identified as alcaligenes castellani and agrobacterium conn respectively,which were able to oxidize arsenite（As（III））into arsenate（As（V））.[Conclusion]The optimal temperature and pH were 30 ℃and 9 respectively for two bacterial strains.In addition,sodium lactate medium concentration had a certain impact to arsenic-oxidizing.