P选择素和ICAM-1在肾缺血再灌注损伤中的作用

Role of P - selectin and ICAM - 1 in Renal Ischemia/Reperfusion injury

目的探讨Ｐ选择素和细胞间粘附分子－１（ＩＣＡＭ－１）在肾缺血再灌注损伤中的作用。方法建立肾缺血再灌注损伤大鼠模型，观察缺血再灌注后肾组织Ｐ选择素和ＩＣＡＭ－１的表达，并用Ｐ选择素单克隆抗体进行了治疗。结果发现缺血再灌注后肾小管上皮细胞空泡变性和片状坏死，血尿素氮（ＢＵＮ）和肌酐（ＳＣｒ）水平升高；但再灌注前５ｍｉｎ经Ｐ选择素单抗处理的动物肾组织与正常相近，肾小管上皮细胞未见变性及坏死，血ＢＵＮ和ＳＣｒ水平明显减低。免疫组化检测提示再灌注后好组织Ｐ选择素和ＩＣＡＭ－１表达增强，单抗治疗组肾组织未见Ｐ选择素和ＩＣＡＭ－１表达。结论Ｐ选择素和ＩＣＡＭ－１与肾缺血再灌注损伤相关，Ｐ选择素单抗对此具有保护作用。

Objective To investigate the role of P- selectin and intercellular adhesionmolecule - 1 (ICAM - 1) in renal ischemia/reperfusion injury. methods Adhesion moleculeexpression was measured by immunohistochemistry and renal function was detected in ratswith renal (I/R) injury. Some I/R rats were treated with anti - P - selectin monoclonalantibody (mAb). Results UP-regulated expression of P - selectin and ICAM - 1 in renaltissue, renal histological lesions and renal failure were present in the rats with I/R injury,while these changes were mild in the mAb treated group. Conclusion The results suggestedthat P - selectin and ICAM - 1 alight contribute to renal I/R injury, and anti - P - selectinmAb might prevent the kidney from I/R injury.