摘要
目的检测实验性糖尿病不同时期系统和肾组织RAS活性变化。方法STZ-糖尿病大鼠随机分两组:糖尿病对照组(DC组)和Captopril治疗组(ACEID组),均予胰岛素治疗以维持中度高血糖,另设正常组(NC组)。病程7、30、90d时测系统和肾内肾素、ACE活性和ATII浓度。结果DC组在7、30d时PRA正常,至90d下降;血浆和肾内ATII、肾内TRA及血清ACE在7、30、90d时均升高。整个实验中ACE变化不明显。而ACEID组血浆ATII、30和90d肾内ACE及30d肾内ATII均明显下降,但90d肾内ATII仍高于NC组。结论糖尿病早期系统和肾组织RAS活性增加;长期ACEI治疗可能激活组织局部合成ATII的旁路途径。
Objective To examine the systemic and renal tissue levels of thecomponents of renin - angiotensin system (RAS) periodically in experimental diabetic rats.Methods The STZ - induced diabetic rats were divided into two groups in random: one wasdiabetic control group (DC group) and the other was treated with captopril (ACEID group).The animals in both groups were all injected with insulin to maintain moderatehyperglycemia. In addition, a relevant normal control group was provided (NC group).Plasma and intrarenal renin activities, ACE activity and AT II levels were measured on the7th,30th,90th day after the onset of diabetes. Results PRA in DC group was not differentcompared to NC group 7 and 30 days after diabetes induction, but was decreased on the 90,,day. Plasma and intrarenal AT II levels and intrarenal renin activity as well as serum ACEactivity were all increased on the 7th, 30th and 90th day after the onset of diabetes. There wereno significant changes in total renal ACE activity throughout this sutdy in DC group ascompared with NC group. On the other hand, Plasma AT II levels throughout this study andintrarenal ACE activity on the 30th and 90th day as well as intrarenal AT II concentration onthe 30th day after induction of diabetes all significantly reduced in ACEID group. But theintrarenal AT II concentration on the 90,h day was still higher than that in NC group despitecaptopril treatment. Conclusion These observations suggest that the systemic andintrarenal tissue RAS activities in early exerimental diabetes are increased. In addition,these results also indicate that the alternative pathways of local tissue AT 11 biosynthesisindependent of ACE might be stimulated in diabetes with chronic ACEI treatment.
出处
《上海第二医科大学学报》
CSCD
1999年第6期515-518,共4页
Acta Universitatis Medicinalis Secondae Shanghai
关键词
糖尿病
肾组织
RAS
diabetes mellitus renin - angiotensin system captopril