胃病患者口腔中的幽门螺杆菌

Helicobacter pylori in the oral cavity of gastric disease patients

目的 通过多聚酶链反应(PCR) 检测胃病患者口腔中的幽门螺杆菌( Hp) 。方法 从13 例有上消化道症状经内镜检查证实的胃病患者采集胃粘膜、口腔含漱液和6 个牙位的龈上及龈下菌斑,应用PCR 检测标本中的Hp 。同时用酶联免疫法(ELISA) 检测静脉血、唾液、龈沟液中抗Hp 特异性IgG 抗体。结果 PCR 检测胃粘膜均为Hp 阳性。6 例患者(46 .2 % ) 的含漱液和11 例患者(84 .6 % ) 的至少一份菌斑样本中检测出Hp 。龈下菌斑中Hp 的检出率(38 .5 % ) 显著高于龈上菌斑(19 .2 % )(P< 0 .01) 。此外, 从患者静脉血、唾液、龈沟液中均可检测到抗Hp 特异性IgG 抗体。结论 本研究结果表明龈沟或牙周袋可能是口腔Hp

Objective To investigate the presence of helicobacter pylori in the oral cavity of gastric disease patients.Methods Gastric biopsies,mouthwash solution,supra and subgingival dental plaque of six teeth were collected from 13 endoscopically diagnosed gastric disease patients.Polymerase chain reaction (PCR) assay was used to detect Hp in these samples.Whole saliva,gingival crevicular fluid (GCF) and sera were obtained for measuraement of Hp-specific IgG antibody by ELISA.Results By PCR amplification,all gastric biopsy specimens (100%) and 6 mouthwash solutions (46.2 %) were Hp-positive.11 out of 13 patients (84.6%) had at least one Hp-positive plaque sample.Statistical analysis indicated the Hp positive rate was significantly higher in subgingival plaque(38.5%)than that in supragingival plaque (19.2 %)(P<0.01).Hp-specific antibody was detected by ELISA in sera, saliva and GCF.Conclusion Gingival crevicular and periodontal pocket may be an important reservoir of Hp.