邻苯二甲酸二乙基己酯(DEHP)胁迫下红鳍笛鲷不同组织生化指标的变化

The Change of Biochemical Indexes in Different Tissue of Crimson Snapper(Lutjanus erythropterus) Exposed to Di-(2-ethylhexyl)phthalate(DEHP)

以红鳍笛鲷(Lutjanus erythropterus)幼鱼为实验生物,根据急性毒性实验结果(96hLC50为10.73mg·L-1)设置邻苯二甲酸二乙基己酯(DEHP)的浓度为0.12、0.60、3.00mg·L-(1以丙酮为对照),在实验进行6、12、24、48h和96h时,分别检测肝脏和鳃组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,以及脑组织乙酰胆碱脂酶(AChE)活性。结果表明,肝脏组织中的SOD反应灵敏且活性明显被诱导,低剂量组(0.12、0.60mg·L-1)SOD活性受到的诱导效应较高剂量组(3.00mg·L-1)更明显;与对照组比较,肝组织中MDA含量在DEHP曝露的12h显著性升高,但48h的MDA含量显著性降低(P<0.01),随曝露时间呈波动变化。鳃组织中的SOD活性明显低于肝脏组织,整个过程中表现为先升高后降低的变化规律,其中高剂量组(3.00mg·L-1)受到的诱导最明显;与对照组比较,MDA含量12h后即显著升高(P<0.01),随后MDA含量开始下降并围绕对照组水平上下波动。各剂量组脑组织AChE活性仅在6h受到抑制,此后受到明显的诱导作用酶活性升高,24h达到最大值,并显著高于对照组(P<0.01),但96h后恢复到对照组水平,呈明显的时间-效应关系。以上结果显示,DEHP对红鳍笛鲷幼鱼组织酶活在实验浓度下影响显著,对水生生物存在危害,应对其生态风险加以关注。

Based on the acute toxicity result,crimson snapper（Lutjanus erythropterus） was exposed to different concentrations of DHEP（0.12,0.60,3.00 mg·L-1,acetone control） and sampled at 6,12,24,48 h and 96 h.Activities of superoxide dismutase（SOD） and malondialdehyde（MDA）contents in liver and gills,and activities of acetylcholinesterase（AChE） in brain were all detected.Results showed that SOD activity in liver was induced significantly compared with the control group（P0.05）,and the low concentration group（0.12 mg·L-1 and 0.60 mg·L-1）was higher than the high concentration group（3.00 mg·L-1）.MDA contents in liver changed with the increase of exposure time,which increased at the exposure time of 12 h and then reduced at 48 h.SOD activity in gills was lower than that in liver,and followed a increase-decrease trend,especially,which was induced significantly at the high concentration of 3.00 mg·L-1.Compared with the control group,MDA contents increased significantly at 12 h（P0.01）,which was then declined to the level of the control group.After the exposure of DEHP,AChE activity was first inhibited at 6 h,then induced significantly and reached the peak level（P0.01）at 24 h,finally recovered to the control level at 96 h.It was concluded that DEHP could affect the enzyme acitivty of crimson snapper.Therefore,more attention of DEHP should be paid to avoid or handle the corresponding risks to aquatic organisms.