纳米金标芯片检测用于前列腺癌多基因诊断的实验研究

Gold Nanoparticles microarray for the diagnosis of prostate cancer experimental study of mutiple genes

目的利用纳米金标基因芯片检测前列腺癌(prostatic cancer,PCa)特异性多基因,探讨其临床意义。方法应用纳米金标基因芯片对11个PCa特异性基因靶标(IGF1、P27、AR、PSMA、KLK3、PCNA、Ki-67、KLK1、KLK2、TMPRSS2、SREBF2)进行检测,对各基因表达进行研究。结果纳米金标基因芯片检测单链核酸的灵敏度达到80f mol/L,在优化条件下,目标上调基因呈阳性表达,下调及阴性对照无显色,可肉眼分辨。结论纳米金标基因芯片技术检测PCa特异性基因方法快速简单,灵敏度高,特异性好,可对选定的11个目标基因定性及半定量检测,不需要借助于特殊仪器,操作简单,样本保存时间长,在普通实验室即可完成。纳米金标基因芯片检测有望成为PCa早期诊断的新一代芯片检测系统。

Objective To detect multiple genes of the prostatic cancer with labelled Nanogold microarray and deal with its clinical significance.Methods Application of the labelled Nanogold microarray to detect 11 kinds of genes for prostate cancer targets（IGF1,P27,AR,PSMA,KLK3,PCNA,Ki-67,KLK1,KLK2,TMPRSS2,SREBF2）,study on each of the gene expressions.Results The sensitivity of dectection of the single-stranded DNA with the method of labelled Nanogold microarray reached to 80 fmol/L,under the condition of optimised,each upregulation gene probe point presented the obvious chromogenic expression.Downregulation gene and the negative control presented no chromogenic expression.This can be identified by naked eyes.Conclusions Utilize the labelled Nanogold microarray to detect multiple genes of the prostatic cancer is simple.It has a high sensitivity and a high specificity.It can achieve a qualitative or semi-quatitative detection without the help of any special instruments for the 11 kinds of genes for prostate cancer targets we has choose.Also,the procedure is easy,which can be done in a normal laboratory,and the sample can be preserved for a long time.So it is expected to be a new generation of the chip detection system on early diagnosis of the prostatic cancer.