大鼠原位膀胱癌模型的建立及CT鉴定方法

Establishment and identification of living body of orthotopic rat model of bladder cancer

目的研究N-甲基亚硝基脲(N-methyl-nitrosourea,MNU)诱导大鼠原位膀胱癌模型的建立及CT鉴定方法。方法 50只SD大鼠随机分为两组,对照组(A组)15只,实验组(B组)35只。A组大鼠经尿道膀胱内灌注生理盐水,每两周一次,每次0.1ml,共4次。B组大鼠用MNU以同样的方法灌注,每次0.2mg。于第14周末对两组大鼠进行膀胱CT扫描诊断,并且处死后取膀胱组织行病理学观察。结果A组13只大鼠经CT扫描未发现膀胱肿瘤,病理检查未见肿瘤组织。B组28只大鼠CT扫描膀胱均见明显异常,表现为膀胱壁局部肿块突起或不规则增厚、密度不均,病理诊断均为膀胱癌。结论MNU诱导大鼠原位膀胱癌模型是一种较为简单、可靠的建模方法。活体大鼠膀胱CT扫描与病理学结果有高度一致性,可作为该模型的活体鉴定方法。

Objective To establish an orthotopic rat model of bladder cancer induced by N-methyl-nitrosourea（MNU）and identification the living body with computed tomography（CT）scan in this experimental model.Methods 50 SD rats were divided randomly into the control group（group A）with 15 rats and experimental group（group B）with 35 rats.Each rat of group A was treated with 0.1 ml normal saline by per urethra administration every other week,totally 4 times.Meanwhile,each rat of group B was treated with 0.2 mg MNU.Then,at the 14th week,all the rats were evaluates by CT scanning and pathological examination.Results No bladder tumor was detected by CT scanning and pathological examination in group A.In group B,abnormal changes were detected in each of the 28 rats by CT scanning,and manifested as local mass,thickening bladder wall accompanied with heterogeneous density,bladder cancer was diagnosed by pathology in each of rats.Conclusions A rat model of orthotopic bladder cancer can be established by per urethra administration of MNU.CT scanning is a reliable diagnostic technique concerning this model.