鱼腥草全草中黄酮的提取和含量测定

Content Determination of Flavonoid in Whole Plants of Houttuynia cordata

以乙醇为溶剂,采用索氏回流法提取鱼腥草全草中的黄酮。优化了索氏回流法的提取温度、乙醇体积分数、提取时间、固液比等提取条件;索氏回流法的最佳提取条件为提取温度100℃、乙醇体积分数80%、提取时间4 h、固液比1∶30(m/V,g∶mL,下同),在此条件下,黄酮提取率为12.59%。采用硝酸铝比色法测定鱼腥草全草中的黄酮含量,结果表明,芦丁浓度在48.75～377.31μg/mL范围内与吸光度呈良好的线性关系(R2=0.999 4);相对标准差RSD为0.21%,加样平均回收率为99.10%,表明该方法重复性良好,结果稳定可靠。

Taking the whole plant of Houttuynia cordata Thunb.as the raw material,ethanol as solvent extraction,Soxhlet extraction method was used to extract flavonoid.The elements such as extraction temperature,ethanol concentration,extraction time,solid-liquid ratio which affected the yield of flavonoid from whole plants of H.cordata were investigated by orthogonal experiment.Content of flavonoid was determined by Al（NO3）3 colorimetry.The results showed that absorption had good linear relationship with rutin concentration at the range of 48.75~377.31μg/mL（R2=0.999 4）.RSD was 0.21%,indicating good repetitiveness.The average recovery rate was 99.10%,it showed that the method was stable and reliable.The optimal conditions were as follows： extraction temperature at 100℃,ethanol concentration of 80%,extraction time 3 h,solid-liquid ratio 1∶30.The yield was 12.59% under these conditions.