四环素类药物抗氧自由基作用的研究

A study of the effects on reactive oxygen species interfering with tetracyclines

目的 比较四环素类抗生素对全血吞噬细胞产生氧自由基（ＲＯＳ） 和非细胞体系产生Ｏ·２ 和·ＯＨ 的影响。方法 应用化学发光方法测定人全血吞噬细胞在受调理酵母多糖诱导下产生的ＲＯＳ，黄嘌呤黄嘌呤氧化酶体系产生的Ｏ·２ 和Ｖｉｔ ＣＣｕＳＯ４酵母多糖Ｈ２Ｏ２ 体系产生的·ＯＨ。结果 四环素类药物均有清除和抑制中性粒细胞产生氧自由基和Ｏ·２ 的作用，但美满霉素作用最强，美满霉素还具有清除·ＯＨ 的作用，而其它四环素类药物无此作用。结论 四环素类药物可清除或抑制吞噬细胞产生的ＲＯＳ和清除Ｏ·２ 。

AIM To study the effects of tetracyclines drugs including tetracycline, minocycline, doxycycline and oxytetracycline on ROS generated by leukocyte and O · 2 ,·OH generated by cell free system. METHODS Chemiluminescence (CL) method was used to measure: ① reactive oxygen species (ROS) generated in whole blood leukocytes induced by opsonized zymosan; ② superoxide ( O · 2 ) induced by xanthine xanthine oxidase system; ③ hydroxy radicals (·OH) generated by Vit C CuSO 4 zymosan system. RUSULTS All drugs of tetracyclines had effects on inhibiting or scavenging ROS generated in leukocyte and O · 2 generated in the cell free system. The effect of minocycline is the strongest of all drugs. Minocycline had an effect on scavenging·OH, but other tetracyclines had not. CONCLUSIONS The all tetracyclines have effects on inhibiting or scavenging ROS generated by leukobytes and O · 2 generated by cell free system.