家蚕细胞基因工程人α干扰素对小鼠的免疫调节作用

The immune regulation of rhIFN α in mice

目的：观察家蚕细胞基因工程α１ 干扰素（ｒｈＩＦＮα１） 对小鼠的免疫调节作用。方法：采用测定环磷酰胺３０ｍｇ／ｋｇ 免疫抑制小鼠碳粒廓清速率，血清溶血素的方法观察ｒｈＩＦＮα１０ ．１ ，０ ．４ ，１ ．２ ×１０６ＩＵ／ｋｇ 对校正廓清指数，脾脏和胸腺重量以及溶血血红蛋白值的影响；利用离体胸腺细胞与ｒｈＩＦＮα１１５０００ ，１５００ ，１５０ＩＵ／ｍｌ 共孵，观察（ｒｈＩＦＮα１）对胸腺细胞的增殖作用。结果：ｒｈＩＦＮα１ 增强免疫抑制小鼠巨噬细胞对碳粒的吞噬作用，使廓清指数增加（ Ｐ＜０ ．０１） ，脾脏重量增加（ Ｐ＜ ０ ．０１） ，血红蛋白值有所增加；但ｒｈＩＦＮα１１ ．２ ×１０６ＩＵ／ｋｇ 却能使正常小鼠的血红蛋白值大幅度增加；ｒｈＩＦＮα１１５００ ，１５０００ＩＵ／ｍｌ 能促使胸腺细胞增殖，且１５０００ＩＵ／ｍｌ 能促进０ ．１ ％ ＰＨＡ 的增殖作用（ Ｐ＜０ ．０５） 。结论：家蚕细胞基因工程人α１

OBJECTIVE:To observe the immunity effects rhIFN α 1(recombined interferon α 1 using with genes of Chinese silkworm) on mice.METHOD:Measuring the serum phagocytic index,weight of the immune organ(spleen and thymus)and hemolytic hemoglobin of rhIFN α 1 0.1,0.4 and 1.2×10 6IU/kg in immunatory inhibited mice with cyclophaphamide(30mg/kg).Observe the proliferation effects of rhIFN α 1 by incubation of thymus cells with rhIFN α 1 of 15000,1500 and 150IU/ml.RESULTS:rhIFN α 1 enhanced the phagocytosis to carbon in immunatory inhibited mice,and increased the serum phagocyte index(P<0.01);it also increased the weight of spleen(P<0.05) and the hemolytic hemoglobin value.rhIFN α 11.2×10 6IU/kg improved the hemolytic hemoglobin greatly in normal mice;rhIFN α 11500,15000IU/ml accelerated the proliferation of lymphocyte in mice,and rhIFN α 115000IU/ml enhanced the proliferation effects of 0.1%PHa(P<0.05).CONCLUSION:rhIFN α 1 showed the immunatory improvement in mice at different aspects