ELISA测定牛奶、猪血浆和猪尿中的磺胺二甲嘧啶

DETERMINATION OF SULPHADIMIDINE RESIDUES IN MILK,SWINE PLASMA AND SWINE URINE BY ELISA

用兔抗磺胺二甲嘧啶抗血清，建立了测定磺胺二甲嘧啶的 Ｅ Ｌ Ｉ Ｓ Ａ 法，测定线性范围为（００５～５０） μｇ／ Ｌ，最低检出浓度为 ００２５ μｇ／ Ｌ。方法回收率全牛奶与脱脂牛奶分别为（８２１％ ～１２８８）％ 和（９０６％ ～１２４０）％ ，猪血浆为（８４９％ ～１０３８）％ ，猪尿为（８９７％ ～１２２４）％ 。方法简便快速，重现性好，检出限低。

A competitive enzyme linked immuno sorbent assay (ELISA) was established with aid of self made rabbit antibody against sulphadimidine(SM 2) for determination of SM 2 in milk,swine plasma and swine urine. The dynamic range of determination was 0.05~5.0 μg/L with a detection limit of 0.025 μg/L,much better than the limit obtained by the analogous literature(1~10 μg/L) and well below the European maximum permitted amount of SM 2 in milk and pork (25 μg/kg). The recovery values obtained by standard addition to whole milk and skimmed milk were found to be in the range of 82.1%~128.8% and 90.6%~124.0%,respectively,to swine plasma to be 84.9%~103.8%,and to swine urine to be 89.7%~122 4%.No positive samples were found from 270 collected milk samples by the developed ELISA method.