实验性消化道溃疡肠上皮内淋巴细胞体外转化能力与IFN_γ分泌活性的变化

The Changes of Proliferation and Interferon-γ Secretion of Intestinal Intraepithelial Lymphocyte from Mice with Experimental Digestive Ulcer

目的：了解消化道溃疡肠上皮内淋巴细胞（ＩＥＬ）的转化及ＩＦＮγ的分泌能力。方法：利用水浸限制刺激诱发的小鼠消化道应激溃疡动物模型，采用〔３ Ｈ〕ＴｄＲ掺入法和ＥＬＩＳＡ法分别检测ＩＥＬ的体外转化能力和ＩＦＮγ的分泌水平。结果：给予小鼠水浸限制刺激诱发消化道溃疡后３ ｄ，ＩＥＬ对有丝分裂原ＣｏｎＡ刺激的体外转化能力最强，刺激指数为１．１５，１ 周后降至正常。此时，ＩＦＮγ的分泌水平也最高，第６ 天尚未恢复到正常水平。结论：在应激性消化道溃疡发生后。

Objective:In order to understand the ability of the proliferation and INF γ secretion of intestinal intraepithelial lymphocytes (IELs) in digestive ulcer. Methods:[ 3H] TdR incorperation and ELISA techniques were used to examine the ability of proliferation and INF γ secreting level of IEL in the animal model with experimental digestive ulcer induced by water immersion restrain stress. Results:The proliferation of the IELs to the stimulation of mitogen ConA was significantly enhanced and the stimulation index was increased to 1.15 on the third day after the stress, and restored to the normal level after a week; The level of IFN γ secreted by the IELs was also the highest on the same day and could not restore completely to the normal even on the sixth day after the stress. Conclusion:The IELs were activated in digestive ulcer and considered to be important in the intestinal mucosa immunity. Conclusion:The IELs were activated in digestive ulcer and considered to be important in the intestinal mucosa immunity. The IELs were activated in digestive ulcer and considered to be important in the intestinal mucosa immunity.