实验性脊髓缺血性损伤的免疫组织化学评价

The Immunohistochemical Evaluation in Experimental Ischemic Spinal Cord Injury

目的：应用免疫组织化学方法观察实验性兔腰髓缺血４０ｍ ｉｎ、再灌流４ ｈ 的脊髓运动神经元胞体和轴突的病理学改变。方法：采用神经元特异性烯醇酶（ＮＳＥ）抗体和神经中丝（ＮＦ）抗体分别特异标记神经元胞体和轴突，结果进行图象分析。结果：缺血４０ ｍ ｉｎ，前角运动神经元胞体和轴突内ＮＳＥ和ＮＦ反应异常地增强，胞体内ＮＳＥ和ＮＦ分布紊乱聚集。再灌流４ ｈ，前角运动神经元胞体和轴突内反应阳性，胞体内分布散乱、稀疏、崩溃和溶解，轴突肿胀、扩大、消失。图象分析前角运动神经元胞体内ＮＳＥ和ＮＦ的面积、灰度和前索内轴突的数量，其结果具有明显的统计学意义。

Objective:The paper was to evaluate possible significances of immunohistological method in assessing experimental ischemic damage on the spinal cord.Methods:Neuron specific endolase(NSE) and neurofilament(NF) antibody were used to specifically label cell bodies and axons of the neuron in the spinal cord,and results were analysed by the methods of the image pattern analyis.Results:The excessive misaccumulation of NSE and NF were observed in the cell bodies and axons of motorneurons in ventral horn after 40 min of ischemia, and the distributions of NSE and NF in the cell bodies were accumulative. The NSE and NF immunohistochemical reaction could be shown in the cell bodies and axons of motorneuorns after 4 h of reperfusion,and the distributions were divergent,rare and disintegrative,the axons were swollen and vanish. The areas,grey levels of NSE and NF in the cell bodies and the numbers of axons in the anterior funiculus were measured and the statistical analysis showed the significance difference.Methods:Neuron specific endolase(NSE) and neurofilament(NF) antibody were used to specifically label cell bodies and axons of the neuron in the spinal cord,and results were analysed by the methods of the image pattern analyis.Results:The excessive misaccumulation of NSE and NF were observed in the cell bodies and axons of motorneurons in ventral horn after 40 min of ischemia, and the distributions of NSE and NF in the cell bodies were accumulative. The NSE and NF immunohistochemical reaction could be shown in the cell bodies and axons of motorneuorns after 4 h of reperfusion,and the distributions were divergent,rare and disintegrative,the axons were swollen and vanish. The areas,grey levels of NSE and NF in the cell bodies and the numbers of axons in the anterior funiculus were measured and the statistical analysis showed the significance difference.Neuron specific endolase(NSE) and neurofilament(NF) antibody were used to specifically label cell bodies and axons of the neuron in the spinal cord,and results were analysed by the methods of the image pattern analyis.Results:The excessive misaccumulation of NSE and NF were observed in the cell bodies and axons of motorneurons in ventral horn after 40 min of ischemia, and the distributions of NSE and NF in the cell bodies were accumulative. The NSE and NF immunohistochemical reaction could be shown in the cell bodies and axons of motorneuorns after 4 h of reperfusion,and the distributions were divergent,rare and disintegrative,the axons were swollen and vanish. The areas,grey levels of NSE and NF in the cell bodies and the numbers of axons in the anterior funiculus were measured and the statistical analysis showed the significance difference.Results:The excessive misaccumulation of NSE and NF were observed in the cell bodies and axons of motorneurons in ventral horn after 40 min of ischemia, and the distributions of NSE and NF in the cell bodies were accumulative. The NSE and NF immunohistochemical reaction could be shown in the cell bodies and axons of motorneuorns after 4 h of reperfusion,and the distributions were divergent,rare and disintegrative,the axons were swollen and vanish. The areas,grey levels of NSE and NF in the cell bodies and the numbers of axons in the anterior funiculus were measured and the statistical analysis showed the significance difference.Conclusion:The pathological changes of cell bodies and axons of motorneurons were demonstrated clearly by immunohistochemical reaction.