子宫内膜癌组织中Bcl-2和ki-67的表达及意义

Significance of Bcl-2 and ki-67 Expression in Endometrial Carcinoma

目的：探讨子宫内膜癌中抗凋亡基因Ｂｃｌ－２和细胞增殖活性指标ｋｉ－６７的表达及其与临床病理因素和预后的关系。方法：采用ＳＰ法免疫组化染色石蜡包埋的子宫内膜癌组织。结果：６１例子宫内膜癌中组织学分级Ⅰ级者较Ⅱ、Ⅲ级子宫内膜癌Ｂｃｌ－２表达显著升高（ Ｐ＜ ０．０１），存活时间超过５年者较５年内死亡者的表达明显增加（Ｐ＜０．０５）。而各临床分期间，合并宫内膜增生与未合并宫内膜增生者间Ｂｃｌ－２表达无明显差异（ Ｐ＞ ０．０５）。６１例子宫内膜癌以单克隆抗体ＭＩＢ－１代表的细胞增殖指数与组织学分级、临床分期均无关。但５年内死亡病例组的平均增殖指数明显高于存活时间超过５年者（Ｐ＜ ０．０１）。Ｂｃｌ－２与ＭＩＢ－１在子宫内膜癌中的表达没有明显的相关性（Ｐ ＞ ０．０１）。结论：Ｂｃｌ－２在从子宫内膜增生、非典型增生到癌的变化过程中逐渐减少，是一个重要的预后因素。

Objective:We investigated the expression of Bcl 2 and ki 67 in endometrial carcinoma and their relationships to clinico pathologic features and prognosis.Methods:Immunohistochemical staining was performed using a SP method.Bcl 2 monoclonal antibody and MIB 1(paraffin ki 67)monoclonal antibody was used respectively in the paraffin embedded specimens of endometrial carcinoma. Results:28 of 61 cases of endometrial carcinoma showed Bcl 2 reactivity with the positive rate of 45.9%.The reactivity of Bcl 2 correlated negatively with the grades of tumor(P<0.01). The group of disease free survival above 5 years has significantly higher expression than group of death within 5 years(P<0.05).There was no significant difference between Bcl 2 expression and clinical stage or with or without endometrial hyperplasia.The proliferative fraction with MIB 1 was 22.16%± 3.88% . It was not associated with histological grade or clinical stage. But the group of death within 5 years has higher expression than group of disease free survival above 5 years(P<0.01).Bcl 2 expression was not related with that of ki 67.Methods:Immunohistochemical staining was performed using a SP method.Bcl 2 monoclonal antibody and MIB 1(paraffin ki 67)monoclonal antibody was used respectively in the paraffin embedded specimens of endometrial carcinoma. Results:28 of 61 cases of endometrial carcinoma showed Bcl 2 reactivity with the positive rate of 45.9%.The reactivity of Bcl 2 correlated negatively with the grades of tumor(P<0.01). The group of disease free survival above 5 years has significantly higher expression than group of death within 5 years(P<0.05).There was no significant difference between Bcl 2 expression and clinical stage or with or without endometrial hyperplasia.The proliferative fraction with MIB 1 was 22.16%± 3.88% . It was not associated with histological grade or clinical stage. But the group of death within 5 years has higher expression than group of disease free survival above 5 years(P<0.01).Bcl 2 expression was not related with that of ki 67.Immunohistochemical staining was performed using a SP method.Bcl 2 monoclonal antibody and MIB 1(paraffin ki 67)monoclonal antibody was used respectively in the paraffin embedded specimens of endometrial carcinoma. Results:28 of 61 cases of endometrial carcinoma showed Bcl 2 reactivity with the positive rate of 45.9%.The reactivity of Bcl 2 correlated negatively with the grades of tumor(P<0.01). The group of disease free survival above 5 years has significantly higher expression than group of death within 5 years(P<0.05).There was no significant difference between Bcl 2 expression and clinical stage or with or without endometrial hyperplasia.The proliferative fraction with MIB 1 was 22.16%± 3.88% . It was not associated with histological grade or clinical stage. But the group of death within 5 years has higher expression than group of disease free survival above 5 years(P<0.01).Bcl 2 expression was not related with that of ki 67.Results:28 of 61 cases of endometrial carcinoma showed Bcl 2 reactivity with the positive rate of 45.9%.The reactivity of Bcl 2 correlated negatively with the grades of tumor(P<0.01). The group of disease free survival above 5 years has significantly higher expression than group of death within 5 years(P<0.05).There was no significant difference between Bcl 2 expression and clinical stage or with or without endometrial hyperplasia.The proliferative fraction with MIB 1 was 22.16%± 3.88% . It was not associated with histological grade or clinical stage. But the group of death within 5 years has higher expression than group of disease free survival above 5 years(P<0.01).Bcl 2 expression was not related with that of ki 67.Conclusion: The expression of Bcl 2 gradually decreased from endometrial hyperplasia, atypical hyperplasia to endometrial carcinoma. It was an important prognostic factor. ki 67 also plays an important role in prognosis.