Vigilin在多种细胞系以及人原发性肝细胞肝癌中的表达研究

Expression of Vigilin in Cell Lines and Human Hepatocellular Carcinoma

目的探讨Vigilin基因在肿瘤细胞系、正常细胞系以及人原发性肝细胞肝癌组织中的表达及其与肝癌发生的关系。方法提取肝癌细胞系、宫颈癌细胞系和正常细胞系总蛋白,采用免疫印迹方法对Vigilin的表达进行半定量检测。收集59例人原发性肝细胞肝癌标本,进行Vigilin的免疫组织化学染色。结果 Vigilin在所检测的细胞系中,除外周血淋巴细胞外的细胞系均有表达,其中肝癌细胞系中Vigilin的表达均强于正常细胞系(P<0.05)。肝癌组织、癌旁组织和远癌组织中大部分细胞均表达Vigilin,其表达量分别为0.2226±0.054、0.2060±0.056和0.1820±0.038(P<0.001)。肿瘤组织Vigilin高表达的比例为54%,而癌旁和远癌组织高表达的比例分别为32%和6%。Vigilin在肿瘤组织中的表达状态和临床参数间均无明显关系。结论 Vigilin高表达与细胞增殖有关,其可能参与人原发性肝细胞肝癌的发生或发展。

Objective To explore the expression status of Vigilin（high density lipoprotein binding protein） in various cell lines and human hepatocellular carcinoma（HCC） tissues.Methods The expression of Vigilin was measured semiquantitatively with western blot in hepatic cancer,cervical cancer and normal cell lines.The samples of 59 hepatocellular carcinoma tissues,59 adjacent liver tissues and 33 distant non-tumor liver tissues were collected,Vigilin expression in the above samples was detected by immunohistochemistry.Results Vigilin expressed in all cell lines,but no expression was found in peripheral blood lymphocytes.The expression levels of Vigilin in tumor cell lines were higher than those in normal cell lines（P〈0.05）.Most of the hepatic cells expressed Vigilin,but the expression levels were different（tumor tissues：0.2226±0.054,adjacent tissues： 0.2060±0.056,distant tissues： 0.1820±0.038,P〈0.001）.Highly expression of Vigilin was observed in 54% of tumor tissues,35% adjacement tissues,and 6% of distant non-tumor tissues,respectively.ConclusionVigilin may have relationship with HCC progression and proliferation.