35例复发麻风患者耐药基因检测

Genic testing for drug - resistance in 35 relapsed leprosy patients

目的:检测麻风菌对氨苯砜(DDS)和利福平(RFP)的耐药。方法:收集临床麻风复发病例皮损组织标本,巢式PCR扩增DDS和RFP耐药基因片段,用直接测序鉴定耐药基因突变。结果:在35例复发病例的皮损组织标本中,共检测出12例DDS耐药基因突变,有5种突变型。但未发现利福平耐药菌株。结论:尽管本研究没有发现利福平耐药菌株,但仍应继续开展对利福平耐药的监测。

Objective： To detect drug resistance in relapsed leprosy patients. Methods： Skin lesions were obtained from relapsed leprosy patients. Dapsone （DDS） and rifampicin （RFP） resistant gene segments were tested by nested PCR, and PCR products were sequenced to identify mutations. Results： DDS resistance was discovered in 12 of 35 re- lapsed leprosy cases with five mutation patterns. No RFP- resistant strain was identified. Conclusion： Monitoring RFP resistance should continue, in spite of the negative result in the current study.