柴胡疏肝散及其拆方对慢性应激抑郁大鼠模型海马组织ERK1/2 mRNA表达的影响(英文)

Effect of Chaihu Shugan San and its components on expression of ERK1/2 mRNA in the hippocampus of rats with chronic mild unpredicted stress depression

目的:探讨柴胡疏肝散及其拆方的抗抑郁作用及机制。方法:将SD大鼠随机分为正常对照组、模型对照组、全方干预组、拆方干预I组和II组、氟西汀对照组。采用慢性轻度不可预见性应激配合孤养复制抑郁模型;运用敞箱试验和蔗糖水消耗实验观察大鼠行为改变;荧光实时定量PCR(FQ-PCR)检测各组大鼠海马组织细胞外调节蛋白激酶(extracellular regulated protein kinases 1/2,ERK1/2)mRNA表达水平。结果:与正常对照组比较,模型组大鼠海马组织ERK1/2 2-ΔΔCT显著降低,差异具有统计学意义(P<0.01),表明模型组海马ERK1/2mRNA表达明显下调。与模型组比较,全方组、氟西汀组大鼠海马组织ERK1/2 2-ΔΔCT均明显升高,差异具有统计学意义(P<0.05或P<0.01);拆方I组ERK1 2-ΔΔCT亦明显上调,差异具有统计学意义(P<0.05);而拆方II组海马组织ERK1/2 2-ΔΔCT均无明显增高,与模型组比较差异无统计学意义(P>0.05)。结论:慢性轻度不可预见性刺激结合孤养可诱导大鼠脑组织ERK1/2mRNA表达下调,柴胡疏肝散可能通过促进海马组织ERK1/2mRNA的表达,改善抑郁大鼠模型的行为学异常,发挥抗抑郁作用;拆方I组也具有较好的抗抑郁作用,但作用不及全方组;拆方II组则无明显抗抑郁作用,但具增强抗抑郁作用。

Objective To explore the antidepressant effect and mechanism of Chaihu Shugan San（CHSGS） composition,a compound traditional Chinese herb medicine and its components.Methods Rats were randomly divided into 6 groups： normal control group,model control group,a CHSGS group,a component I group,a component II group and a fluoxetine control group.The depression model was replicated by chronic unpredictable mild stress and single house for 28 d.Behavioral scores of the rats were detected by Open-field test and sucrose solution consumption test,and ERK1/2 mRNA expression in the hippocampus tissue was assayed by real-time fluorescent quantitative PCR.Results ERK1/2 mRNA expression was down-regulated in the depression model group compared with the normal control group（P0.01）.Compared with the model group,ERK1/2 mRNA expression in the CHSGS group and fluoxetine group was both up-regulated（P0.05 or P0.01）;and only ERK1 mRNA expression in the component I group was up-regulated（P0.05）.No significant difference existed between the component II group and the model group（P0.05）.Conclusion Isolated-living condition and chronic mild unpredictable stress can down-regulate the expression of ERK1/2 mRNA in the hippocampus tissue.CHSGS may exert an antidepressant effect through increasing the expression of ERK1/2 mRNA in the hippocampus,component I may play an important role in its antidepression effect,while compatibility of the use of component II can enhance the antidepressant efficacy.