颗粒细胞凋亡及线粒体膜电位与卵巢功能的相关性研究

The correlation between ovarian function and granulosa cells apoptosis as well as mitochondria membrane potential

目的探讨人卵巢黄素化颗粒细胞的凋亡率及线粒体膜电位的变化与卵巢功能及妊娠结局的相关性。方法应用流式细胞仪AnnexinV-PI双染法检测颗粒细胞凋亡,同时利用新型荧光探针JC-1测定细胞线粒体跨膜电位,分析其与卵巢功能的关系。结果黄素化颗粒细胞的凋亡率与年龄、基础FSH水平呈正相关(P<0.01),与获卵数,HCG日雌二醇水平及优质胚胎率呈负相关(P<0.01),颗粒细胞的凋亡率与Gn支数差异无统计学意义(P>0.05)。妊娠组颗粒细胞的凋亡率低于未妊娠组(P<0.05)。黄素化颗粒细胞线粒体膜电位与颗粒细胞凋亡率呈显著负相关(r=0.634,P<0.01),与优质胚胎率及妊娠率呈正相关关系(r=0.460,P<0.05)。结论颗粒细胞的凋亡和线粒体膜电位可能影响卵巢功能及胚胎的体外发育潜能,从而影响妊娠结局。

Objective To investigate the apoptotic rate of human ovary luteinization granulosa cells and the correlation between the changes of mitochondria membrane potential and ovarian function as well as pregnancy outcome.Methods The apoptotic rate of granulosa cells was detected quantitatively by flow cytometry with AnnexinV-FITC and propidium iodide staining method.The mitochondria membrane potential was observed by flow cytometry with the fluorescence dye JC-1.Results The apoptotic rate of human ovary luteinization granulosa cells was positively correlated with patients＇ age and basic levels of serum FSH（P0.01）,however,which was negatively related with the number of oocytes retrieved,serum E2 levels at the day of HCG and high quality embryo rates（P0.01）,and there was no significant correlation between apoptotic rate of granulosa cells and total amount of gonadotropins administration（P0.05）.The apoptotic rate in pregnancy group was significantly lower than that in non-pregnancy group（P0.05）.With the increase of apoptotic rate of granulosa cells,the mitochondria membrane potential was decreased（P0.01）.Mitochondria membrane potential on human ovary luteinization granulosa cells was negatively correlated with apoptotic rate of granulosa cells,however,which was positively correlated with pregnancy rates and high quality embryo rates（P0.05）.Conclusion The granulosa cells apoptosis and mitochondria membrane potential may affect ovarian function as well as the potentiality of development of embryo in vitro,as a result,which may influence pregnancy outcome.