酶解辅助乙醇法提取土茯苓总黄酮条件研究

Study on Extraction Process of Total Flavonoids from Smilax Glabra by Enzyme-assisted Hydrolysis

本文采用植物复合酶酶解辅助乙醇法提取土茯苓总黄酮。以芦丁为标准品,用铝离子显色、紫外-可见分光光度法测定总黄酮含量。采用单因素实验探讨酶添加量、酶解时间和酶解pH值对总黄酮提取率的影响。结果表明:最佳提取条件为酶添加量1.0%,酶解时间3.0h和酶解pH值5.0;最佳条件下,粗提物干粉得率13.9%,干粉中总黄酮含量4.0%,总黄酮提取率为0.53%。

The total flavonoids were extracted from smilax glabra with enzyme-assisted hydrothermal method.The content of the total flavonoids was measured by UV-vis spectrophotometry under assisited with aluminum ion colour using Rutin as standard.Extraction ratio of total flavonoids was investigated as a main target.The effects of enzyme added volume,enzyme hydrolysis time and media pH value on the extraction rate were discussed.The results showed that the optimum extract conditions are as follow：when enzyme concentration is 1.0%,hydrolysis time is 3.0 h,enzymatic hydrolysis media pH value is 5.0,the powder ratio of crude extraction is 13.9%,and the content of total flavonoids in this powder is up to 4.0 % with the extract ratio of total flavonoids being 0.53%.