RNA干扰抑制FAK基因表达对人胰腺癌细胞凋亡及吉西他滨敏感性的影响

Enhances gemcitabine chemosensitivity and apoptosis of human pancreatic carcinoma cells after decreasing FAK level by RNA interference

目的 探讨RNA干扰技术抑制黏着斑激酶（FAK）基因表达增强人胰腺癌PANC-1细胞对化疗药物敏感性及凋亡能力的研究.方法 针对FAK mRNA序列设计合成短发夹状干扰RNA（siRNA）的DNA模板,构建pRNAT-FAK重组表达载体,转染人胰腺癌PANC-1细胞;通过RT-PCR分析其对PANC-1细胞内源性FAK表达的影响;激光共聚焦显微镜检测PANC-1细胞凋亡的形态学改变;用四甲基偶氮唑蓝法观察PANC-1细胞对化疗药物吉西他滨敏感性的改变;采用分光光度计检测 Caspase 活性.结果 成功构建 pRNAT-FAK重组质粒,并成功转染PANC-1细胞;RT-PCR证实重组质粒在mRNA显著抑制FAK基因表达（P＜0.01）;吉西他滨组及吉西他滨加pRNAT-FAK组细胞则检测出明显的细胞凋亡;四甲基偶氮唑蓝结果证明在和吉西他滨联合作用下,pRNAT-FAK组PANC-1细胞的生长抑制率明显增高（P＜0.01）;Caspase 3活性明显高于空白对照绀和阴性对照组.结论 pRNAT-FAK可抑制FAK在人胰腺癌PANC-1细胞中的表达,并增强PANC-1细胞对吉西他滨敏感性.

Objective To explore the relationship between the expression of FAK and Gemcitabine Chemosensitivity and Apoptosis of human pancreatic carcinoma PANC-1 cells by decreasing FAK gene expression by RNA interference. Methods One pair of DNA template coding siRNA was synthesized against PANC-1 to reconstruct pRNA-FAK,which was transfected into PANC-1 cells. The FAK expression in PANC-1 ceils were transfected with pRNAT-FAK, and it was detected by RT-PCR. MTT was used to observe the growth inhibiting ratio induced by gemcitabine in PANC-1 cells. Cell apoptosis was analyzed by fuorescence microscopy. Caspase-3 activity was measured by spectrofluorometer. Results RT-PCR analyses demonstrated that pRNAT-FAK could significantly inhibit the expression of FAK in PANC-1 cells （P 〈 0. 01 ） ;after PANC-1 cells were exposed to Gemeitabine,the growth inhibiting ratio, Caspase-3 activity and cell apoptosis of pRNAT-FAK transfected cells was significantly increased compared to that of control group and negative group（ P 〈 0.01 ）. Conclusion pRNAT- FAK could significantly inhibit the expression of FAK,increase Chemosensitivity and apoptosis of PANC-1 cells.