某地区急性缺血性脑梗死患者GPⅠbα基因VNTR多态性研究

Investigation of VNTR polymorphism on GPⅠbα gene of patients with acute ischemic cerebral infarction in some district

目的探讨GPⅠbαVNTR基因多态性与脑梗死的相关性。方法应用聚合酶链反应-限制性片段长度多态性分析、DNA测序分析技术,对119例急性缺血性脑梗死患者和117例健康对照者进行血小板膜糖蛋白(GP)Ⅰbα基因可变数目串联重复序列(VNTR)分析。结果该地区汉族人群中不仅存在B、C、D等位基因和CC、DD、CD、BC、BD基因型,而且还发现了A等位基因和AC基因型;其中以C、D等位基因和CC、CD基因型为主;急性缺血性脑梗死病例组血小板膜GPⅠbαVNTR CC基因型和CD基因型与健康对照组相比,差异有统计学意义(P<0.05)。结论该地区汉族人血小板膜GPⅠbαVNTR CC基因型和CD基因型与缺血性脑梗死显著相关。

Objective To study the correlation between variable number of tandem repeats （VNT/~） polymorphism on platelet membrane glycoprotein（GP） I ba gene and cerebral infarction （CI）. Methods VNTR polymorphism on GP ] ba gene of 119 cases of patients with acute ischemic cerebral infarction and 117 cases of healthy controls were analyzed by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） analysis and DNA sequencing. Results /3, C, D allele and CC, DD, CD, BC, BD genotype were detected in Han people of Kunming district. An allele and AC genotype were also detected. Among these alleles and genotypes, the mainly detected were C, D allele and CC, CD genotype. There was statistically significant difference in the fre quency of CC and CD genotype between patients with acute ischemic CI and the controls （P〈0.05）. Conclusion The CC and CD genotype of the VNTR polymorphism on GPIba gene is significantly associated with ischemic CI in Han people of Kunming dis- trict.