美洛昔康对Aβ诱导阿尔茨海默病大鼠脑内一氧化氮的作用

Effect of Meloxicam on the nitric oxide induced by Aβ in model rat with Alzheimer's disease

目的:研究美洛昔康对β-淀粉样蛋白(Aβ)诱导的阿尔茨海默病(AD)模型大鼠脑内一氧化氮(NO)的作用,并探讨其抑制炎症作用的机制。方法:采用Aβ1-40海马注射建立AD大鼠模型。术后第2天各组大鼠灌胃给药,连续30d。美洛昔康小、中、大剂量分别为:1、2、4mg/(kg·d)。生理盐水(NS)对照组及模型对照组分别给予等容量NS及0.5%羧甲基纤维素钠溶液。电迷宫刺激器检测大鼠学习记忆能力;生化法检测脑组织中NO的含量变化。结果:美洛昔康能提高AD大鼠的学习记忆能力;抑制AD大鼠脑内的表达。结论:美洛昔康可能通过抑制NO的分泌,改善大鼠的学习记忆能力。

Objective：To investigate the effect and mechanism of meloxicam on the nitric oxide induced by beta amyloid protein in model rats with Alzheimer＇s disease（AD）.Methods：The rat model was established by the microinjection of Aβ1-40 into hippocampus.The drugs were given intragastrically daily for 30 days,the day after operation.The rats of meloxicam groups took different doses[1,2 and 4 mg/（kg·d）].The rats of normal saline control group and AD model group took the same volume of normal saline and 0.5% sodium carboxymethyl cellulose.The capacity for learning and memorizing was tested on rats with electrical maze.The expression of nitric oxide was tested by biochemical method.Results：The capacity for learning and memorizing in AD model would be enhanced by meloxicam,and the expression of nitric oxide would be decreased.Conclusion：Meloxicam may ameliorate the pathological change in AD model rat＇s brain through inhibiting the expression of nitric oxide.