急性肾炎患儿尿微量白蛋白,Kappa轻链与临床关系的研究

Research on the relationship among urine ALB,Kappa light chain and clinical features in acute nephritis children

目的:研究急性肾炎患儿尿微量白蛋白(ALB)、Kappa轻链与临床之间的关系,寻找新的肾损害敏感无创伤指标。方法:分别使用尿ALB酶免疫法一步法和闪射比蚀法检测临床收集的标本中的尿ALB、Kappa轻链、Lambda轻链的含量,研究急性肾炎患儿尿AlB,Kappa轻链急性期、恢复期与对照组的比较以及与临床的关系。结果:急性肾炎患儿尿ALB急性期与恢复期及对照组比较差异有统计学意义(P<0.01);尿ALB恢复期与对照组比较差异无统计学意义(P>0.05);ALB急性肾炎患儿尿Kappa链急性期与恢复期及对照组比较差异有统计学意义(P<0.01);尿Kappa链恢复期与对照组比较差异无统计学意义(P>0.05);急性肾炎患儿Lambda链急性期、恢复期与对照组比较差异无统计学意义(P>0.05)。急性肾炎患儿尿ALB与补体C3、C4、血尿在统计学上无相关性(R=0.186、0.032、0.128,P>0.05),ALB与尿蛋白有正相关(R=0.71,P<0.05)。尿Kappa链与补体C3、C4、血尿在统计学上无相关性(R=0.023、0.01、0.21,P>0.05),与蛋白尿呈正相关(R=0.69,P<0.05)。结论:急性肾炎患儿尿ALB、Kappa轻链可作为新的肾损伤敏感无创伤指标,早期发现及早干预,对肾炎的预后具有重要意义。

Objective：To study the relationship among urine ALB,KAPPA light chain and clinical features in acute nephritis children and find a new non-invasive index sensitive to kidney damage.Methods：The content of urine ALB,Kappa light chain and lambda light chain of the collected specimens was tested with urinary ALB（albumin） and one-step enzyme immunoassay and Shanshe assay respectively,to research their urine ALB,Kappa light chain at acute stage and convalescence and their relationship with the clinical features and to compare them with those in the control group.Results：Compared with that in control group,the difference of urine ALB in acute nephritis children at acute stage and recovery was more significant（P0.01）,but at recovery stage there was no significant difference in both groups（P0.05）.Comparing urinary Kappa chain of acute nephritis children at acute and recovery stages with that in the control group,there were significant differences（P0.01）,but at recovery stage in both groups urinary Kappa chain had no significant difference（P0.05）.Lamda chain in acute and recovery phases of both groups had no significant difference（P0.05）.Urine ALB showed no correlation with complements C3,C4 and hematuria（R=0.023,0.01,0.21,P0.05）.Urine ALB was positively correlated with urinary protein in acute nephritis（R=0.71,P0.05）.Urine Kappa chain showed no correlation with complements C3,C4 and hematuria（R=0.023,0.01,0.21,P0.05）.Urine Kappa was positively correlated with proteinuria（R=0.69,P0.05）.Conclusion：Acute nephritis urinary ALB,Kappa light chain can be used as kidney damage sensitive indicators of the new non-invasive.Early detection and early intervention are of great significance for the prognosis of nephritis.