中孕羊水细胞培养和染色体制备方法的分析

Analysis of amniotic fluid cells culture in mid-pregnant and chromosomal preparation method

目的探讨一种高效、简便和稳定的羊水细胞染色体制备方法,提高产前诊断的准确性和成功率。方法选择2002年9月3日到2009年7月9日孕周为16.0～29的产前筛查为高风险的孕妇2689位,抽取羊水进行细胞培养,行羊水细胞染色体核型分析。结果 2689例羊水细胞培养成功率为99.9%,核型分析的成功率为99.8%,发现异常核型74例,异常核型率为2.8%,其中18-三体10例,占0.37%,21-三体19例,占0.71%。结论本方法具有高效、简便、染色体带纹清晰等优点,在产前诊断中具有结果准确可靠和实用价值。

Objective：To investigate a high effective,simple and steady amniotic fluid cells chromosomal preparation method,and to improve veracity and achievement ratio of prenatal diagnose.Methods：We cultured amniotic fluid cells and analysised them by chromosome karyotypes from 2689 prenatal-screening high risk women with 16.0～29 pregnant weeks since September 3,2002 to July 9,2009.Results：We found that the succeed proportion of 2689 amniotic fluid cells cultivate is 99.9%,the succeed proportion of karyotypes analysis is 99.8%,74 abnormal chromosomal karyotypes were detected,the abnormal tate was 2.8%,including 10 trisomy 18（0.37%）,19 trisomy 21（0.71%）.Conclusions：The method possessed many advantages such as high effect,simpleness and distinct karyotypings,and it has veracity and practicality in prenatal diagnose.