高效液相色谱法测定枸橼酸钾溶液中枸橼酸钾的含量

Determination of Potassium Citrate in Potassium Citrate Solution by High Performance Liquid Chromatography

目的建立高效液相色谱法测定枸橼酸钾溶液中枸橼酸钾含量的方法。方法色谱柱采用十八烷基硅烷键合硅胶为填充剂（150 mm×4.6 mm,5μm）,以0.025 mol/L磷酸氢二钠（用磷酸调pH值为2.5）溶液为流动相,流速：1 ml/min,检测波长：210 nm。结果以峰面积（X）对进样浓度（Y）线性回归,回归方程为Y=0.3764X-25.45,r=0.9999,线性范围429.4~1288μg/ml,枸橼酸钾回收率为99.4%,RSD为1.3%。结论本方法操作简便,测定结果准确,重复性好,可用于枸橼酸钾溶液中枸橼酸钾的含量测定。

Objective To develop an high performance liquid chromatography quantitative method for the determination of potassium citrate in potassium citrate solution.Methods The chromatographic conditions include column C18（150 mm ×4.6 mm,5 μm） and a mobile phase of 0.025 mol/L disodium hydrogen phosphate（adjusted to pH 2.5 by phosphoric acid）.The flow rate was 1 ml/min and monitored at 210 nm.Results The regression equation for potassium citrate was Y=0.3764X-25.45,r=0.9999 and the linear range was 429.4-1288 μg/ml.The average recovery of potassium citrate was 99.4% and RSD was 1.3%.Conclusion This method is simple,time-saving and accurate.It can be used for routine analysis of potassium citrate in potassium citrate solution.