母爱剥夺对大鼠海马PP1Cγ表达与神经发育的影响

Effects of maternal deprivation on hippocampal PP1Cγ gene expression and neurobehavior of rats TU

目的探讨母爱剥夺（MD）对PP1Cγ的表达改变和神经发育的影响。方法新生雄性仔鼠随机分为MD组（35只）和正常对照组（24只），MD组在出生后1～21d每天接受3h的MD。通过早期神经行为实验观察神经发育情况，水迷宫实验观察学习记忆的影响，实时荧光定量PCR分析PP1Cγ的基因表达改变。结果MD能改变大鼠体质量、上肢运动协调能力和大鼠痛觉敏感性[（26．23±2．81）g，（30．38±3．85）g；（19．37±11．89）s，（22．39±17．62）s；（4．36±1．76）s，（5．26±2．55）s；P〈0．05]，对神经反射影响较小[（0．83±0．30）s，（0．83±0．34）s；（3．68±1．63）s，（5．60±3．01）s；（3．00±0．00），（3．00±0．00）；P〉0．05]，对学习记忆能力影响不明显（P〉0．05）。在出生后第22天MD可致海马PP1Cγ表达下降[（2．19±0．62），（3．52±0．86）；P〈0．05]，在出生后60d大鼠PP1Cγ表达无明显改变[（1．73±0．78），（1．33±0．34）；P〉0．05]，而在出生后第90天海马PP1Cγ表达升高[（2．85±0．34），（1．34±0．93）；P〈0．05]。结论MD可致大鼠早期神经行为和海马PP1Cγ的表达发生改变，但对学习记忆的影响却很小。同时PP1Cγ表达随生长发育时期的不同而改变。

Objective To investigate the effect of maternal deprivation （MD） on neurnbehavior and PP1Cγ gene expression in hippocampus. Methods Male pups were randomly divided into MD group（thirty-five） and control group（twenty-four）. From PND 1 to PND 21 ,pups in the MD groups underwent daily maternal deprivation for 3 h （ Postnatal day ）. Neurobehavior was observed to investigate neurodevelopmeut, Morris water maze was used to measure spatial learning and memory, and Real-Time quantitative PCR was employed to analyze PP1Cγ gene expression. Results Several significant deficiencies were observed in bodyweight and grasping reflex while a great enhancement in hot-plate test in rat pups suffering from MD （ （26.23 ± 2.81 ） g vs. （ 30.38 ± 3. 85 ） g; （ 19.37 ± 11.89 ） s vs. （22.39 ± 17.62 ） s ; （4.36 ± 1.76 ） s vs. （ 5.26 ± 2.55 ） s ; P 〈 0.05 ）, but deficiencies in neurological reflexes were subtle（ （ 0.83 ± 0.30 ） s vs. （ 0.83 ± 0.34 ） s ; （ 3.68 ± 1.63 ） s vs. （ 5.61 ± 3.01 ） s ; （3.00 ± 0.00） vs. （ 3.00 ± 0.00） ; P 〉 0.05 ）. MD had a subtle influence on spatial learning and memory （P 〉 0.05 ）. Meanwhile, MD could lead to PP1Cγ expression down-regulation on PND 22 （ （2.19 ± 0.62） vs. （3.52 ± 0.86） , P 〈 0.05） which was in line with early neurobehavior results. No difference was found compared with MD group and control group on PND60 （ （ 1.73 ± 0.78 ） vs. （ 1.33 ± 0.34） ; P 〉 0.05 ）. However,there was the up-regulation of PP1Cγ expression on PND 90 （ （2.85 ± 0.34） vs. （ 1.34 ± 0.93 ） ; P 〈 0.05 ）. Conclusion MD alters early neurobehavior and hippoeampal PP1Cγ gene expression in the Wistar rats ,but has a subtle effect on learning and memory. At the same time,MD can make PP1Cγ expression in the hippoeampus varying with the age.