不同HPV感染状态宫颈癌细胞中INK4a表达研究

Expression of INK4a in Human Cervical Carcinomas Cell Lines with Different HPV Infection Status

[目的]探讨不同HPV感染状态的宫颈癌细胞中INK4a基因的表达情况。[方法]提取细胞总RNA,应用逆转录—聚合酶链反应(RT-PCR)技术检测宫颈癌细胞CaSki(HPV16感染)、HeLa(HPV18感染)、C-33A(无HPV感染)中INK4a表达水平。[结果]不同HPV感染状态的宫颈癌细胞CaSki(0.3967±0.0491),HeLa(0.5567±0.1037),C-33A(0.5633±0.0722)中INK4amRNA表达无明显差异(P>0.05)。[结论]不同HPV感染状态的宫颈癌细胞中INK4a表达规律趋于一致。检测INK4a基因表达对于宫颈癌的早期诊断和筛查具有重要意义。

[Purpose] To investigate INK4a expression in human cervical carcinoma cell lines with different HPV infection status.[Methods] Total RNA was isolated from human cervical carcinomas cell lines CaSki（HPV16 infection）,HeLa（HPV18 infection） and C-33A（no HPV infection）.The mRNA expression of INK4a gene was determined by RT-PCR.[Results] There was no significant difference among CaSki（0.3967±0.0491）,HeLa（0.5567±0.1037） and C-33A（0.5633±0.0722） INK4a mRNA expression（P0.05）.[Conclusions] INK4a expression in cervical carcinoma cells accords with different HPV infection status.Detection of INK4a gene expression plays an important role in screening and early diagnosis for cervical cancer.