加兰肽及其受体在人嗜铬细胞瘤中的表达及意义

Expression of Galanin and Galanin Receptors in Human Pheochromocytoma and its Significance

[目的]探讨加兰肽(GAL)及其受体(GALR1、GALR2)在嗜铬细胞瘤中的表达及意义。[方法]分析功能不同嗜铬细胞瘤患者临床特点,ELISA法检测功能组(n=130)、功能隐匿组(n=26)、高血压组(n=30)和健康组(n=30)的血清GAL水平;免疫组化法检测GAL、GALR1和GALR2的表达水平。[结果]功能隐匿组与功能组患者年龄、性别、肿瘤性质、部位、肿瘤数目及多巴胺、尿皮醇水平均无显著性差异,两组24h肾上腺素、去甲肾上腺素水平有显著性差异(P<0.001)。功能组、功能隐匿组、高血压组和健康组的血清GAL浓度无显著性差异。功能组、功能隐匿组GAL阳性表达率分别为94.6%和23.1%(P<0.001),两组GALR1阳性表达率分别为95.4%和96.2%(P>0.05),两组GALR2阳性表达率分别为86.9%和34.6%(P<0.001)。[结论]嗜铬细胞瘤中分泌儿茶酚胺功能与患者血清GAL浓度无关,可能与肿瘤组织中GAL及GALR2的表达有关。

[Purpose] To investigate the expression of galanin（GAL） and galanin receptors（GALR1,GALR2） in human pheochromocytoma.[Methods] The clinical features of pheochromocytoma with different function were analyzed.The serum GAL levels of function group（n=130）,function hidden group（n=26）,high BP group（n=30） and heathy group（n=30） were detected by ELISA.Expression of GAL,GALR1 and GALR2 was detected by immunohistochemical method.[Results] There was no significant difference in age,gender,the nature of tumor,location,number of tumors,dopamine and urinary free cortisol（UFC） levels between function hidden group and function group,but 24h epinephrine,norepinephrine levels were significantly different between the 2 groups.The serum GAL levels among function group,function hidden group,high BP group and heathy group was not significantly different.The positive rate of GAL in function group and function hidden group was 94.6% and 23.1%,respectively（P0.001）.The positive rate of GALR1 in the 2 groups was 95.4% and 96.2%（P0.05）,and the positive rate of GALR2 in the 2 groups was 86.9% and 34.6%,respectively（P0.001）.[Conclusions] The function of secrete the catecholamine in pheochromocytoma does not correlate with the level of serum GAL,it is possible correlate with the expression of GAL and GALR2 in the tumor.