基于SSR分子标记的龙井群体种的遗传多样性及遗传分化研究

Genetic Diversity and Differentiation of Longjing Tea Population Based on SSR Markers

利用48对SSR引物对来自龙井群体种的5个不同居群的91份材料进行了遗传多样性和遗传分化研究。结果表明,龙井群体有较高的遗传多样性水平,多态信息含量PIC在0.0567～0.7476之间,平均为0.4382,其中有16个位点属于高度多态位点,30个位点属于中度多态位点。哈迪-温伯格法则(Hardy-Weinberg)平衡检验结果表明,33.3%的位点符合Hardy-Weinberg平衡,66.7%的SSR位点不符合Hardy-Weinberg平衡。AMOVA分析表明,不同群体之间所贡献的变异百分比为3.45%,个体间的变异百分比为94.98%。龙井群体的遗传分化程度较低,个体间遗传分化系数FIT为0.0502,群体之间的遗传分化系数FST仅为0.0345。

The genetic diversity and differentiation of 91 individuals from five populations of Longjing tea landrace were characterized by 48 pairs of SSR markers.It was showed that the level of genetic diversity within Longjing tea population was high.The polymorphism information content（PIC） was ranging from 0.0567 to 0.7476 and the average PIC was 0.4382.Among 48 SSR sites in study,16 was high polymorphism,30 was medium polymorphism.Hardy-Weinberg equiliberum（HWE） test showed that 33.3% SSR sites were in accord with the HWE and 66.7% sites were not in HWE.AMOVA analysis showed that percentage variation among populations was 3.45% and percentage variation within individuals was 94.98%.The genetic differentiation was lower in Longjing tea population,the fixation indices within individuals and among populations was 0.0502（FIT） and 0.0345（FST） respectively.