蕲艾提取液对大鼠肝组织细胞周期蛋白Cyclin D1表达的影响

Effect of Artemisia argyi extract solution on expression of Cyclin D1 in rat with liver fibrosis

[目的]研究蕲艾提取液对免疫性肝纤维化大鼠肝组织细胞周期蛋白Cyclin D1表达的影响。[方法]72只Wister大鼠随机分为正常对照(正常)组,肝纤维化模型(模型)组,蕲艾提取液低、中、高剂量组和丹参组。猪血清腹腔内注射法构建肝纤维化大鼠模型。采用荧光定量PCR和Western blot法检测大鼠肝脏组织细胞周期蛋白Cyclin D1蛋白和mRNA表达水平。[结果]与模型组比较,蕲艾提取液组可下调Cyclin D1 mRNA及蛋白表达(P<0.05,<0.01)。[结论]蕲艾提取液可下调Cyclin D1的表达,发挥对细胞周期的调控,使细胞复制停滞于G0/G1期,抑制细胞增殖,进而达到抗肝纤维化的作用。

[Objective]To investigate the effect of Artemisia argyi extract solution on the expression of Cyclin D1 in rat with hepatic fibrosis.[Methods]Seventy-two wister rats were randomly divided into normal control group,liver fibrosis model group,Artemisia Argyi extracting solu tion low,middle and high-dose group,and the Salvia group.Rat liver fibrotic models were established by intraperitoneal injection with pig serum.The expression of Cyclin D1 mRNA and protein was detected by RT-PCR and western blot respectively.[Results]Artemisia argyi extract solution group could down-regulate the expression of Cyclin D1 mRNA and protein compared with liver fibrosis model group（P0.05,P0.01）.[Conclusion]Artemisia argyi extract solution get down the expression of Cyclin D1,playing on cell cycle regulation,which maybe one of the mechanisms of anti-liver fibrosis.